Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (37): 6851-6854.doi: 10.3969/j.issn.1673-8225.2011.37.003

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Tumor necrosis factor-alpha influences cbfa1/runx2 gene expression in mouse osteoblasts

Zhu Jian-hua1, Zhang Qin1, Liu Ji-guang2   

  1. 1Stomatological Hospital, Jiamusi University, Jiamusi  154007, Heilongjiang Province, China
    2Department of Science and Technology, Jiamusi University, Jiamusi  154007, Heilongjiang Province, China
  • Received:2011-03-09 Revised:2011-04-20 Online:2011-09-10 Published:2011-09-10
  • Contact: Liu Ji-guang, Doctor, Professor, Department of Science and Technology, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China Liujg5550@163.com
  • About author:Zhu Jian-hua, Professor, Stomatological Hospital, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China zhu8622877@yahoo.com.cn
  • Supported by:

    a grant from Science and Technology Program of Education Department of China, No. 205041*

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Abstract:

BACKGROUND: Tumor necrosis factor-alpha (TNF-α) can decrease alkaline phosphatase (ALP) activity in periodontal ligament fibroblasts and inhibit the functional transformation between periodontal ligament fibroblasts and osteoblasts.
OBJECTIVE: To investigate the effects of TNF-α on the growth of mouse osteoblasts and cbfa1/runx2 gene expression.
METHODS: Well growing mouse osteoblast line MC3T3/E1 were interfered with 20, 40, 60, 80 μg/L TNF-α. The normally cultured cells served as controls. cbfa1/runx2 mRNA expression in osteoblast line MC3T3/E1 was detected by RT-PCR. ALP activity was determined by PNPP method and cell viability was measured by MTT assay.
RESULTS AND CONCLUSION: cbfa1/runx2 mRNA expression was observed in the normally cultured osteoblast line MC3T3/E1. With increasing concentration of TNF-α concentration, cbfa1/runx2 mRNA expression, MC3T3/E1 cell viability and ALP activity were gradually decreased. Results suggested that TNF-α can inhibit osteoblast line MC3T3/E1 growth and cbfa1/runx2 may be involved in osteoblast differentiation.

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