Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6789-6792.doi: 10.3969/j.issn.1673-8225.2011.36.032

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Isolation, culture and identification of rat embryonic mesencephalic dopaminergic neurons

Wei Xiao-bing1, Deng Xing-li1, Wang Ying-li2, Yang Zhi-yong2, Li Zhi-gao2, Liu Ru-en3, Li Yu1   

  1. 1Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming  650032, Yunnan Province, China
    2Department of Cadres Health, First People’s Hospital of Yunnan Province, Kunming  650032, Yunnan Province, China
    3Department of Neurosurgery, China-Japan Friendship Hospital, Beijing  100029, China
  • Received:2009-10-15 Revised:2011-07-30 Online:2011-09-03 Published:2011-09-03
  • Contact: Li Yu, Master, Associate professor, Associate chief physician, Master’s supervisor, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
  • About author:Wei Xiao-bing★, Studying for master’s degree, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China 875112608@qq.com Deng Xing-li☆, Doctor, Lecturer, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China duetch13071@yahoo.com.cn Wei Xiao-bing and Deng Xing-li contributed equally to this paper.
  • Supported by:

    the Science Research Foundation of Yunnan Education Bureau, No. 09Y0153*

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Abstract:

BACKGROUND: Nerve cell transplantation therapy is one of the most promising methods to cure Parkinson’s disease.
OBJECTIVE: To investigate the culture, isolation and identification of rat embryo mesencephalic dopaminergic neurons.
METHODS: The mesencephalon was dissected from rat embryo at embryonic day 14 (E14). The brain tissue was triturated into a fine single-cell suspension. The cells were cultured with the medium containing Neurobasal, B27 supplement, β-mercaptoethanol and fetal bovine serum. The cells were identified with RT-PCR as well as immunocytochemistry, respectively.
RESULTS AND CONCLUSION: The cells survived well in the medium. RT-PCR and immunocytochemistry results showed mostly all neurons expressed molecular markers for dopaminergic neurons. The dopaminergic neurons derived from rat embryonic mesencephalon were successfully isolated and cultured, which will provide the foundation for the further research about cell therapy of Parkinson’s disease.

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