Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6679-6682.doi: 10.3969/j.issn.1673-8225.2011.36.007

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Experimental study on bone marrow mesenchymal stem cells inhibiting T lymphocytes proliferation in patients with aplastic anemia

Liu Zeng-hui1,2, Xiao Yang1, Jiang Zu-jun1, Li Li1, Gao Yang1, Li Yong-hua1, Li Li1, Wang Yao-chun1, Kuang Li-ping1   

  1. 1Department of Hematology, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou  510010, Guangdong Province, China
    2Guangzhou University of Chinese Medicine, Guangzhou  510405, Guangdong Province, China
  • Received:2011-02-19 Revised:2011-04-20 Online:2011-09-03 Published:2011-09-03
  • Contact: Xiao Yang, Master, Department of Hematology, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China jdxiao111@163.com
  • About author:Liu Zeng-hui★, Master, Department of Hematology, General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China; Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China 10304254@qq.com

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Abstract:

BACKGROUND: Previous studies have found bone marrow mesenchymal stem cells (BMSCs) have immunosuppressive effects. T lymphocytes in patients with aplastic anemia (AA) are abnormal activatory and proliferous.
OBJECTIVE: To study the inhibitory effects of BMSCs on T lymphocyte proliferation in vitro in AA patients.
METHODS: T lymphocytes were isolated from the peripheral blood of patients with AA and stained with carboxyfluorescein diacetate succinimidyl ester (CFDA SE), and then co-cultured with BMSCs at 1:3 ratio using closing-contact and Transwell methods. Phytohemagglutinin was added to stimulate T lymphocytes proliferation. Besides, negative and positive controls were set.
RESULTS AND CONCLUSION: Flow cytometry was used to detect the T cell proliferation. CFDA SE staining analysis showed that T lymphocyte proliferation in closing-contact group was much lower than positive control (P < 0.01). T lymphocyte proliferation in Transwell group was lower than positive control (P < 0.05). Besides, T lymphocyte proliferation in closing-contact group was much lower than Transwell group (P < 0.01). BMSCs can inhibit abnormal proliferation of T cells in patients with AA possibly through closing contact and secreting some cytokines, which makes it possible to correct the immune abnormalities in AA. Furthermore, the closing-contact mechanism may play a major role in inhibiting T cell proliferation.

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