Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6661-6664.doi: 10.3969/j.issn.1673-8225.2011.36.003

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Mesenchymal stem cells can differentiate to epidermal cells and express keratins in vitro 

Li Ling-yun1, Du Rong2, Fan Xi-ming1   

  1. 1The 8650 Troop Hospital of Chinese Armed Police Forces, Jinzhong  030600, Shanxi Province, China
    2Outpatient Department of Shanxi Armed Police Forces, Taiyuan  030012, Shanxi Province, China
  • Received:2011-03-08 Revised:2011-04-18 Online:2011-09-03 Published:2011-09-03
  • Contact: Fan Xi-ming, Associate chief physician, the 8650 Troop Hospital of Chinese Armed Police Forces, Jinzhong 030600, Shanxi Province, China
  • About author:Li Ling-yun★, Master, Attending physician, the 8650 Troop Hospital of Chinese Armed Police Forces, Jinzhong 030600, Shanxi Province, China 527031068@qq.com

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Abstract:

BACKGROUND: Animal experiments have proved that bone marrow mesenchymal stem cells (MSCs) can mostly differentiate into epidermal cells.   
OBJECTIVE: To investgate the feasibility of inducing MSCs into epidermal cells and the expression of keratins.
METHODS: MSCs were extracted by Ficoll-Paque density gradient centrifugation. The expressions of CD34 and CD44 in MSCs cultured for three passages were deteced by immunocytochemical staining and flow cytometric examinations. The third passage MSCs were culured in 30% conditioned medium and induced to differentiate into epidermal cells. The changes, of cytokeratin 19 were detect by immunocytochemical staining and the morphological features were observed after being induced.
RESULTS AND CONCLUSION: The MSCs extracted by Ficoll-Paque density gradient centrifugation were pure. After being induced with conditioned medium, cytokeratin 19 in MSCs were observed, indicating that MSCs may be induced into epidermal cells．

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