Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (33): 6231-6234.doi: 10.3969/j.issn.1673-8225.2011.33.037

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In vitro culture and identification of osteoblasts

Wang Yong-ping, Liao Yi, Jiang Yao   

  1. Department of Orthopedics, Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai  200233, China
  • Received:2011-02-09 Revised:2011-04-24 Online:2011-08-13 Published:2011-08-13
  • Contact: Jiang Yao, Chief physician, Professor, Doctoral supervisor, Department of Orthopedics, Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China jiangyao195106@ 163.com
  • About author:Wang Yong-ping☆, Studying for doctorate, Attending physician, Department of Orthopedics, Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China wangyp312@163. com

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Abstract:

BACKGROUND: The methods of osteoblast culture in vitro have certain limitations.
OBJECTIVE: To summarize osteoblast source, in vitro culture method, culture condition, and identification signs.
METHODS: A computer-based retrieval was performed in PubMed and Wanfang databases to search manuscripts published between 2000 and 2010 with the key words” osteoblast, cell culture, identification” in English and Chinese languages. The papers describing in vitro culture and identification of osteoblasts were included. Repetitive research and Meta analysis were excluded.
RESULTS AND CONCLUSION: With the development of cell culture techniques in vitro, osteoblasts from animal bone, periosteum, bone marrow and non-bone tissue have been successfully cultured. Studies have shown that these osteoblasts have typical characteristics and good biological properties. Currently, the cultured osteoblasts are common experimental model in vitro to study physiology, pathology, and repair and have become the basis for studying osteoblast growth and metabolism and bone tissue engineering.

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