Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (33): 6216-6219.doi: 10.3969/j.issn.1673-8225.2011.33.033

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Effects of Bizhongxiao decoction on activation of phosphorylated p38 and JNK proteins in synovium of rats with collagen-induced arthritis

Xiao Yu-mei, Xiong Xin-gui, Chen Jiang, Liang Qing-hua, Guo Ya-jing, Xu Ze-lin, Wu Dan, Fan Rong   

  1. Department of Integrated Traditional and Western Medicine, Xiangya Hospital, Central South University, Changsha   410008, Hunan Province, China
  • Received:2011-03-05 Revised:2011-06-11 Online:2011-08-13 Published:2011-08-13
  • Contact: Liang Qing-hua, Professor, Doctoral supervisor, Department of Integrated Traditional and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China Lqhxy@126.com
  • About author:Xiao yu-mei,★ Studying for master’s degree, Department of Integrated Traditional and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China 179423557@qq.com
  • Supported by:

    Ph.D. Program Foundation of Ministry of Education of China, No.200805330011*; Science and Technology Planning Program of Hunan Province, No.2009SK3163*; Traditional Chinese Medicine Foundation of Hunan Province, No. 2010038*

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Abstract:

BACKGROUND: Reports have shown that abnormal synovial cell signaling is one of important mechanisms of rheumatoid arthritis.
OBJECTIVE: To investigate the effects of Bizhongxiao decoction on activation of phosphorylated p38 and JNK proteins in mitogen-activated protein kinase (MAPK) pathway in synovium of rats with collagen-induced arthritis.
METHODS: A total of 72 Sprague-Dawley rats were randomly and evenly divided into three groups: normal, model, and Bizhongxiao decoction. In the model and Bizhongxiao decoction groups, rat models of collagen-induced arthritis were created by injection of typeⅡcollagen and complete Freud’s adjuvant into rat toes. After 10 days, rats were immunized again. From day 14 after primary immunization, the Bixiaozhong decoction group rats were intragastrically administered Bizhongxiao decoction every day, the model group rats were identically given distilled water, and the normal group rats were allowed to drink water freely.
RESULTS AND CONCLUSION: Western blot analysis results showed that phosphorylated p38 and JNK expressions were significantly greater in the collagen-induced arthritis rats than in the normal controls (P < 0.05). Compared with the model group, phosphorylated p38 and JNK expressions were significantly downregulated in the Bizhongxiao decoction group (P < 0.05). These findings suggest that Bizhongxiao decoction can inhibit phosphorylated p38 and JNK expressions in rheumatoid arthritis.

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