Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (33): 6187-6190.doi: 10.3969/j.issn.1673-8225.2011.33.026

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Fibronectin and integrin alpha 5 expression during the development of mouse renal tubule

Yan Li-jing1, Tian He2, Guo Min2   

  1. 1 Microscope Centre，Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
    2 Department of Histology and Embryology, Liaoning Medical University, Jinzhou  121001, Liaoning Province, China
  • Received:2011-02-19 Revised:2011-06-01 Online:2011-08-13 Published:2011-08-13
  • Contact: Guo Min, Doctor, Professor, Master’s supervisor, Department of Histology and Embryology, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China mguo1118@yahoo. com.cn
  • About author:Yan Li-jing★, Master, Experimentalist, Microscope Centre，Liaoning Medical University, Jinzhou 121001, Liaoning Province, China yanlijing_88@yahoo.com.cn
  • Supported by:

    Education Department Foundation Program of Liaoning Province, No. L20100274*, L20100269*

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Abstract:

BACKGROUND: There are few studies describing interaction of fibronectin and integrin α5 influencing the development of renal tubules. 
OBJECTIVE: To observe the expression of fibronectin and its receptor integrin α5 during the development of mouse renal tubules.
METHODS: Kunming mice at embryonic days 12, 14, 16, 18 and postnatal days 1, 3, 7, 14, 21, 28 and 40 were selected.
RESULTS AND CONCLUSION: Immunohistochemical staining showed that fibronectin expression was expressed in the basement membrane of ureteric bud at embryonic 12 days and then in the basement membrane of renal tubules at any stage.  Integrin α5 began to express at embryonic 12 days in the epithelial cells of the renal tubules. Stereological measurements showed that the surface density of fibronectin and the volume density of integrin α5 increased gradually with the nephrogenesis. Western blot analysis showed that firbonectin and integrin α5 contents increased gradually with kidney development. These results demonstrate that fibronectin and intergrin α5 expression has certain chronological and spatial sequence during the development of mouse renal tubules, which suggest that they play an important role in the development of renal tubules.

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