Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (33): 6182-6186.doi: 10.3969/j.issn.1673-8225.2011.33.025

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Effects of exercises on mitochondrial permeability transition pore, and bcl-2 and bax mRNA expression in rat skeletal muscle

Wang Dong-mei1, Qi Zheng-tang2, Ding Shu-zhe2   

  1. 1Department of Sport and Body Science, Taishan Medical College, Tai’an 271016, Shandong Province, China
    2College of Physical Education & Health, East China Normal University, Shanghai  200241, China
  • Received:2011-02-09 Revised:2011-03-31 Online:2011-08-13 Published:2011-08-13
  • Contact: Ding Shu-zhe, Doctor, Professor, Doctor’s supervisor, College of Physical Education & Health, East China Normal University, Shanghai 200241, China szding@tyxx.ecnu. edu.cn
  • About author:Wang Dong-mei★, Master, Lecturer, Department of Sport and Body Science, Taishan Medical College, Tai’an 271016, Shandong Province, China wangdm72@126. com

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Abstract:

BACKGROUND: Exercises influence the apoptosis of skeletal muscle cells and mitochondrial pathway is one of important pathways mediating cellular apoptosis.
OBJECTIVE: To investigate the effects of exercises on mitochondrial permeability transition pore, and bcl-2 and bax mRNA expression in rat skeletal muscle.
METHODS: Twenty-four Sprague-Dawley male rats were randomly divided into three groups: the control group, 6-week swimming training group (six swimming trainings per week) and one-off exhaustive swimming group (one-off exhaustive swimming exercise). The opening state of mitochondrial permeability transition pore in skeletal muscle was examined with ultraviolet spectrophotometer, and the mRNA expression of bcl-2 and bax in rat skeletal muscle was determined by reverse transcription-polymerase chain reaction.
RESULTS AND CONCLUSION: 6-week swimming training resulted in significant increase of bcl-2 mRNA expression and significant decrease of bax mRNA expression, as well as significant increase of bcl-2/bax mRNA(P < 0.01), but the opening state of mitochondrial permeability transition pore was not altered obviously, compared with the control group. The opening state of mitochondrial permeability transition pore was significantly increased (P < 0.01), bcl-2 mRNA expression was significantly decreased, bax mRNA expression was significantly increased, bcl-2/bax mRNA expression was significantly decreased in the one-off exhaustive swimming group than in the control group (P  < 0.01). These findings suggest that exercise training can regulate skeletal muscle cell apoptosis by altering the opening stage of mitochondrial permeability transition pore and regulating bcl-2/bax mRNA expression in rat skeletal muscle.

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