Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (33): 6085-6089.doi: 10.3969/j.issn.1673-8225.2011.33.002

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Fluoride influences transduction pathway of transforming growth factor superfamily members expressed in osteoblasts

Zhao Yang1, He Tie-ying2, Zhang Ya-lou3   

  1. 1Department of Burn and Plastic Surgery, 2Department of Pancreatic Surgery, First Affiliated Hospital, Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China; 3Department of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-03-05 Revised:2011-06-21 Online:2011-08-13 Published:2011-08-13
  • Contact: Zhang Ya-lou, Doctor, Assistant Researcher, Department of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China zhangyalou12@ yahoo.com.cn
  • About author:Zhao Yang☆, Studying for doctorate, Department of Burn and Plastic Surgery, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China gorgezy@163.com He Tie-ying, Studying for doctorate, Department of Pancreatic Surgery, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China tietie5309@163.com Zhao Yang and He Tie-ying contributed equally to this paper and were considered as co-first authors.
  • Supported by:

    the National Natural Science Foundation of China, No. 30860249*

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Abstract:

BACKGROUND: Transforming growth factor (TGF) beta superfamily members are involved in bone turnover process in fluorosis, but the mechanism remains poorly understood.
OBJECTIVE: To investigate the effects of fluoride on TGF-β1, BPM-2 and SMAD4 signal transduction pathway of osteoblasts cultured in vitro.
METHODS: Human osteoblasts were cultured in vitro and then grouped according to (NaF) dose used: 0 (control), 0.625, 1.250, 2.500, 5.000, 10.000, 20.000, 40.000, 80.000, 160.000 mg/L．Cells were collected after 24 hours of culture, RNA extracted, and the mRNA expression of TGF-β1, BPM-2 and SMAD4 was detected using fluorescent quantitative reverse transcription polymerase chain reaction.
RESULTS AND CONCLUSION: Fluoride may affect mRNA expression of TGF-β1, BPM-2 in osteoblasts and their expression level was related to fluoride concentration. When NaF was 0.625 mg/L, NaF effect on osteoblasts was regulated via TGF-β1, BPM-2 and SMAD4 signal transduction pathway. When NaF was higher or lower than 0.625 mg/L, NaF effect on osteoblasts was not related to TGF-β1, BPM-2 and SMAD4 signal transduction pathway. There may be other transduction pathways involved in this process.

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