Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (33): 6081-6084.doi: 10.3969/j.issn.1673-8225.2011.33.001

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Collagen and tissue inhibitor of metalloproteinase-3 expression in human osteochondral allograft in storage media

Yang Jian1, Huang Wei1, K-L Paul Sung2, 3, Liang Xi1, Chen Hao1, Chen Rong-fu1, Xie Jing2   

  1. 1Department of Orthopedics, First Afiliated Hospital of Chongqing Medical University, Chongqing 400016, China
    2College of Bioengineering, Chogqing University, Chongqing 400030, China
    3Departments of Orthopedics and Bioengineering, University of California, Califonia CA 92093-0412, USA
  • Received:2011-05-06 Revised:2011-07-07 Online:2011-08-13 Published:2011-08-13
  • Contact: Huang Wei, Doctor, Professor, Chief surgeon, Master’s supervisor, Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China 15465950@qq.com
  • About author:Yang Jian★, Master, Surgeon, Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • Supported by:

    a Key Program of Bureau of Health of Chongqing, No. 2010-1-12*

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Abstract:

BACKGROUND: It has been well known that mechanical loading could affect articular cartilage; however, the effects of mechanical loading on articular cartilage stored in tissue media have rarely been reported so far.
OBJECTIVE: To investigate the effects of loading environment on osteochondral allografts stored in tissue culture media.
METHODS: Normal human osteochondral allografts were divided into three groups: control group, in which fresh grafts were used; common group, in which the osteochondral allografts were stored using common tissue culture media; mechanical loading group, in which osteochondral allografts stored in common tissue culture media were given a persistent periodical mechanical loading by a shaking table as a dynamic loading device. 
RESULTS AND CONCLUSION: At 2 weeks after storage, western blot analysis and transmission electron microscopy showed that the dynamic loading condition kept tissue inhibitor of metalloproteinase-3 expression and collagen fibril ultrastructure in articular cartilage significantly better than in static condition (P < 0.01). The results showed that the loading condition benefits the chondrocyte function and collagen ultrastructure in articular cartilage of human osteochondral allograft in storage media. The dynamic noncontact loading may provide a better method in storing human osteochondral allografts.

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