Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (16): 2929-2932.doi: 10.3969/j.issn.1673-8225.2011.16.018

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Function and metabolic activity of hepatocytes cultured by spherical porous chitosan microcarriers

Zhang Rui1, Han Bao-san2, Peng Cheng-hong2   

  1. 1Department of Tumor Surgery, First Affiliated Hospital, Inner Mongolia Medical College, Hohhot  010059,  Inner Mongolia Autonomous Region, China
    2Organ Transplantation Center of Ruijin Hospital Affiliated to Medical College of Shanghai Jiao Tong University, Shanghai  200025, China
  • Received:2010-11-30 Revised:2011-03-01 Online:2011-04-16 Published:2013-11-11
  • About author:Zhang Rui☆, Doctor, Associate chief physician, Department of Tumor Surgery, First Affiliated Hospital, Inner Mongolia Medical College, Hohhot 010059, Inner Mongolia Autonomous Region, China ruizhang999@sohu.com
  • Supported by:

    the National High-Tech Research and Development Plan of China ("863" Program), No. 2007AA02Z487*; the National Natural Science Foundation of China, No. 20074031*, 30672043*, 30772105*

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Abstract:

BACKGROUND: Hepatocyte culture is the key to the hepatocyte transplantation and BAL study. It has turned into the first problem how to get adequate numbers, better activity and well-functioning hepatocyte. In the recently years, the microcarrier as a technique for high density cell culture has been applied in the in vitro culture of hepatocytes.
OBJECTIVE: To detect the function and metabolic activity of human hepatocytes L-02 cultured by spherical porous chitosan microcarriers at each time.
METHODS: The function, including aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) levels, and metabolic activity, including albumin (ALB), urea, glucose contents, of human hepatocytes L-02 cultured by spherical porous chitosan microcarriers were detected at 1, 2, 3, 4, 5 days of culture.
RESULTS AND CONCLUSION: In the two groups, the levels of AST, ALT and LDH decreased at 1-3 days, and increased at 4 days; the contents of ALB, urea and glucose increased at 1-3 days, and decreased at 4 days. The levels of all the indexes in the experimental group were obviously higher than those in the control group (P < 0.05). It indicated that the metabolic activity of human hepatocytes L-02 in the experimental group was higher than that in the control group.

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