Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (16): 2925-2928.doi: 10.3969/j.issn.1673-8225.2011.16.017

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Preparation of drug-loaded poly (D, L-lactide-co-glycolide) nanoparticles and characterization of release behavior in vitro

Xu Wei-hua, Liu Jie, Jin Cheng, Zhang Fu-qin, Dou Ke-feng   

  1. Department of Hepatobiliary Surgery, Xijing Hospital, the Fourth Military Medical University, Xi’an  710032, Shaanxi Province, China
  • Received:2010-10-28 Revised:2010-12-30 Online:2011-04-16 Published:2013-11-11
  • Contact: Dou Ke-feng, Professor, Doctoral supervisor, Department of Hepatobiliary Surgery, Xijing Hospital, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China gdwkgwx@fmmu.edu.cn
  • About author:Xu Wei-hua★, Studying for master’s degree, Department of Hepatobiliary Surgery, Xijing Hospital, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81000987*

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Abstract:

BACKGROUND: As a new drug delivery carrier, medical nanoparticle has currently become the focus of research in the field of medicine.
OBJECTIVE: To construct antitumor drug 5-fluorouracil (5-FU) loaded nanoparticles using the biodegradable material poly (D, L-lactide-co-glycolide) (PLGA).
METHODS: The PLGA nanoparticles were prepared by double emulsification solvent evaporation technique. The morphology and particle size distribution of nanoparticles were determined by field emission scanning electron microscope and laser particle size analyzer, respectively, and the granulation rate of nanoparticles was calculated. The drug loading, encapsulation efficiency of 5-FU and release in vitro were detected by ultraviolet spectrophotometer.
RESULTS AND CONCLUSION: The nanoparticles were spherical in shape with average diameter (186±14) nm. The granulation rate, drug loading and encapsulation efficiency was 70.8%, 6.6%, 28.1%, respectively. The nanoparticles exhibited initial burst release in vitro, releasing a cumulative 36.2% of 5-FU in 24 hours. The cumulative release reached 83.6% at the 10th day. The results suggested that the 5-FU-loaded PLGA nanoparticles were prepared successfully and have slow release function in vitro.

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