Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (16): 2895-2900.doi: 10.3969/j.issn.1673-8225.2011.16.011

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Self-assembling peptide nanofiber scaffold combined with RhoA-siRNA for repair of spinal cord injury

Jiang Yan-wen1, Zhang Wei-wei1, Zhan Xiao-duo1, Kang Xiao-ning1, Dai Xiang2, Zhang Wen-ying3, Liu Zhong-ying1, Dong Wei-ren1, Guo Jia-song1   

  1. 1Department of Histology and Embryology, School of Basic Medical Science, Southern Medical University, Guangzhou  510515, Guangdong Province, China
    2Guangzhou Da'an Clinical Laboratory Center, Guangzhou  510000, Guangdong Province, China
    3Training Institute, Nairuier Body Sculpting and Cosmetic Co., LTD., Guangzhou  511434, Guangdong Province, China
  • Received:2011-01-14 Revised:2011-02-28 Online:2011-04-16 Published:2013-11-11
  • Contact: Guo Jia-song, PhD Professor, Doctoral supervisor, Department of Histology and Embryology, School of Basic Medical Science, Southern Medical University, Guangzhou 510515, Guangdong Province, China jiasongguo@yahoo.com.cn
  • About author:Jiang Yan-wen★, Studying for master’s degree, Department of Histology and Embryology, School of Basic Medical Science, Southern Medical University, Guangzhou 510515, Guangdong Province, China jywen1209@163.com
  • Supported by:

    National Natural Science Foundation of China, No. 30973095*

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Abstract:

BACKGROUND: Up-expression of RhoA after spinal cord injury is one of main reasons of neural regeneration failure. Based on our previous report that self-assembling peptide nanofiber scaffold (SAPNS) could effectively promote the repair of structure and function after spinal cord injury, the complex of SAPNS and RhoA-siRNA was constructed to repair the injured spinal cord.
OBJECTIVE: To explore the promotion of SAPNS-mediated siRNA interference RhoA expression on repair of spinal cord injury.
METHODS: A total of 54 Kunming mice were randomly divided into 4 groups: sham group, saline group, SAPNS group and siRNA+SAPNS group. After transection spinal cord injury model was prepared by removed 1mm spinal cord tissue, saline, SAPNS or the complex of siRNA+SAPNS was filled into the lesion cavities in saline group, SAPNS group and siRNA+SAPNS group. Spinal cord was exposed in sham group.Then, the siRNA transfection efficiency was detected by FAM fluorescent signal; the RhoA expression was detected by immnohistochemistry; the axonal regeneration was examined by neurofilament (NF) immnohistochemistry and behavioral test.
RESULTS AND CONCLUSION: After transplantation of FAM-siRNA+SAPNS, the FAM fluorescent signal could be detected in the nerve fiber in the spinal cord and the neuronal bodies in motor cortex, which indicated the siRNA could be released from the complex and successfully entered into the target cells. Compared with the SAPNS group and saline group, RhoA-siRNA+SAPNS transplantation could significantly reduce the RhoA expression in the neurons, increase the densities of NF positive nerve fiber in the spinal cord injury area, and improve the function of the hindlimbs of the spinal cord injured mice. The results indicated that mediated by SAPNS, RhoA-specific siRNA can effectively interfere the expression of RhoA, in order to promote the repair of spinal cord injury.

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