Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (16): 2867-2870.doi: 10.3969/j.issn.1673-8225.2011.16.005

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Biological stability of long-term culture chondrocytes on three-dimensional culture system of multifactorial sodium alginate

Zhu Zhen-zong, Liang Wei-guo, Shen Yan, Yang Xiao-hong, Dai Li-bing, Tan Jian-rong, Liang Pei-hong, Zhang Jin-li, Kang Ning   

  1. The Fourth Affiliated Hospital, College of Medicine, Jinan University, Guangzhou Red Cross Hospital, Guangzhou Institute of Traumatic Surgery, Guangzhou  510220, Guangdong Province, China
  • Received:2011-01-17 Revised:2011-02-20 Online:2011-04-16 Published:2013-11-11
  • Contact: Liang Wei-guo, Chief physician, Professor, The Fourth Affiliated Hospital, College of Medicine, Jinan University, Guangzhou Red Cross Hospital, Guangzhou Institute of Traumatic Surgery, Guangzhou 510220, Guangdong Province, China liangweiguo@tom.com
  • About author:Zhu Zhen-zong★, Studying for master’s degree, Physician, The Fourth Affiliated Hospital, College of Medicine, Jinan University, Guangzhou Red Cross Hospital, Guangzhou Institute of Traumatic Surgery, Guangzhou 510220, Guangdong Province, China zhuzhenzogn@163.com
  • Supported by:

    Health Commission of Guangdong Province, No. 2004553*; the Key Project of Guangzhou Health Bureau, No. 2004Z005*

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Abstract:

BACKGROUND: Previous studies demonstrated that the survival time of chondrocytes is prolonged in three-dimensional culture system of multifactorial sodium alginate; extracellular matrix has a strong secretion.
OBJECTIVE: To observe the effect of biological stability of long-term culture chondrocytes on three-dimensional culture system of multifactorial sodium alginate.
METHODS: Chondrocytes were implanted on three-dimensional culture system which comprises sodium alginate, hyaluronic acid, chitosan, fibronectin, and basic fibroblast growth factor (bFGF), and the plane culture as controls. The morphological changes, differences of cell growth curves, and the contents of glycosaminoglycan (GAG) of cells supernatant fluid of chondrocytes were regularly observed in two different culture systems.
RESULTS AND CONCLUSION: Morphous of chondrocytes on three-dimensional culture system of multifactorial sodium alginate grew well and flourishingly, and formed the globular group of cells, cell proliferation was active. Proliferation of chondrocytes on the culture system of multifactorial sodium alginate was slightly higher than that implanted on plane cultured 2 days. Proliferation of chondrocytes was significantly higher cultured 4 days on the culture system of multifactorial sodium alginate. Chondrocytes were keep stable proliferation at 6-14 days culture; while the proliferation of chondrocytes cultured on plane was gradually decrease. The contents of GAG of cells supernatant fluid of the three-dimensional culture system were significantly higher than monolayer culture system. Chondrocytes on three-dimensional culture system of multifactorial sodium alginate can long-term culture and keep their biological stability.

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