Abstract:

BACKGROUND: The composite skin developed by using tissue engineering and cell culture technology is still not an ideal artificial skin, which is limited by weak local skin, poor tensile strength and resistance to friction and no skin appendages.
OBJECTIVE: To observe the effect of basic fibroblast growth factor and vitamin C epidermal cell composite transplantation on full-thickness skin defects and to investigate the optimal time promoting the epidermal growth.
METHODS: A total of 36 New Zealand white rabbits were prepared for three full-thickness skin defect models at the back. The self-made amnion carrying composite with basic fibroblast growth factor and vitamin C, pure amnion carrying composite, pure capping were implanted into the three defects, and were randomly divided into three groups. Epidermal cells were implanted into the site in related groups after 7, 14, and 21 days. After 7 days, general observation, histological observation, immunohistochemical observation were carried out.
RESULTS AND CONCLUSION: The groups with autologuos epidermal cells implantation at 21 days had a better effect that those with autologous epidermal cells implantation at 14 and 7 days. Self-made amniotic carrier complex with basic fibroblast growth factor and vitamin C was superior to pure amnion carrying composite and pure capping. Basic fibroblast growth factor and vitamin C promoted the regeneration and renascence of the dermis. The latter of the dermis repairing and the former of the dermis restoring were the best stage which accelerates the renascence and regeneration of the epidermis.