Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 1969-1972.doi: 10.3969/j.issn.1673-8225.2011.11.017

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Effects of serum containing Zaohuang mixture on the expression of connective tissue growth factor in human mesangial cells cultured in vitro

Zhang Li-mei1, He Yu-zhen1, Yin Yan-zhi2, Mu Shou-han2, Ju Jian-wei2, Lü Cong-kui2, Li Du-jun2   

  1. 1College of Traditional and Western Medicine, Binzhou Medical University, Binzhou  264003, Shandong Province, China
    2Department of Nephrology, Hospital of Yantai Economic and Technical Development Zone, Yantai  264006, Shandong Province, China
  • Received:2010-10-09 Revised:2010-12-03 Online:2011-03-12 Published:2011-03-12
  • Contact: Ju Jian-wei, Master, Professor, Chief physician, Department of Nephrology, Hospital of Yantai Economic and Technical Development Zone, Yantai 264006, Shandong Province, China jujianwei1234@sina.com
  • About author:Zhang Li-mei★, Studying for master’s degree, College of Traditional and Western Medicine, Binzhou Medical University, Binzhou 264003, Shandong Province, China zhanglimei6416@ 126.com
  • Supported by:

    the Subject of Yantai Economic and Technical Development Zone*

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Abstract:

BACKGROUND: Connective tissue growth factor (CTGF) is a kind of downstream effector of transforming growth factor-β1. The abnormal expression of CTGF plays an important role in pathological changes of diabetic kidney disease (DKD).
OBJECTIVE: To investigate the effect of Zaohuang mixture contained serum (ZHM) on the expression of CTGF in human mesangial cells (HMC).
METHODS: Serums containing different concentration of ZHM and haikunshenxi were prepared by serum pharmacology method. HMC were incubated in DMEM medium with 10% contained serum and high glucose for 48 hours. The level of CTGF protein and mRNA expression was measured by immunocytochemistry and RT-PCR respectively.
RESULTS AND CONCLUSION: With the stimulation of high glucose, the expression of CTGF protein and mRNA was increased significantly (P < 0.01), which then were decreased by the treatment of ZHM and showed a dose-dependent manner. Compared with haikunshenxi group, CTGF protein and mRNA expression in the ZHM high-dosage group was not statistically significant (P > 0.05). The expression of CTGF in HMC may be induced by high glucose and ZHM can inhibit the expression for prevention and treatment of DKD.

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