Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (10): 1841-1844.doi: 10.3969/j.issn.1673-8225.2011.10.028

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CD28 mRNA expression in peripheral blood mononuclear cells of patients with acute myeloid leukemia

Wang Ya-nan1, 2, Shi Jin-fang1, Gu Guo-hao1   

  1. 1Department of Clinical Laboratory, First Affiliated Hospital of Soochow University, Jiangsu Province Laboratory of Clinical Immunology, Suzhou  215006, Jiangsu Province, China
    2Suzhou Municipal Hospital, Affiliated Hospital of Nanjing Medical University, Suzhou  215002, Jiangsu Province, China
  • Received:2010-11-11 Revised:2010-12-15 Online:2011-03-05 Published:2011-03-05
  • Contact: Gu Guo-hao, Chief physician, Master’s supervisor, Department of Clinical Laboratory, First Affiliated Hospital of Soochow University, Jiangsu Province Laboratory of Clinical Immunology, Suzhou 215006, Jiangsu Province, China guguohao@yahoo.com.cn
  • About author:Wang Ya-nan★, Studying for master’s degree, Department of Clinical Laboratory, First Affiliated Hospital of Soochow University, Jiangsu Province Laboratory of Clinical Immunology, Suzhou 215006, Jiangsu Province, China; Suzhou Municipal Hospital, Affiliated Hospital of Nanjing Medical University, Suzhou 215002, Jiangsu Province, China wyn80520@yahoo.com.cn

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Abstract:

BACKGROUND: Recent studies have found that there is difference in costimulatory molecule CD28 protein expression on the surface of peripheral blood T cells in tumor patients, which suggested that abnormal costimulation pathway may be associated with the occurrence and progress of malignant tumor.
OBJECTIVE: To investigate the costimulatory molecule CD28 mRNA expression in peripheral blood mononuclear cells of acute myeloid leukemia patients.
METHODS: A total of 80 patients with acute myeloid leukenmia patients were selected, including 7 cases of M0 type, 6 cases of M1 type, 18 cases of M2 type, 15 cases of M3 type, 17 cases of M4 type, 9 cases of M5 type, and 8 cases of M6 type. According to acute leukemia curative effect criteria, 80 patients were assigned to complete healing group, remission group and non-remission group. Using Taqman probe, real-time fluorescent quantitation polymerase chain reaction was used to detect CD28 mRNA expression in peripheral blood mononuclear cells of 80 patients and 76 healthy persons.
RESULTS AND CONCLUSION: CD28 mRNA expression was lower in the M1, M3, M4 subtype in peripheral blood mononuclear cells of patients with acute myelocytic leukemia compared with healthy population (P < 0.05). CD28 mRNA expression in non-remission group was lower than healthy population (P < 0.05). No significant difference in CD28 mRNA expression was detected between the complete healing and remission groups and healthy population. Results suggested that acute myeloid leukemia patients possess defects in peripheral blood mononuclear cells CD28 mRNA expression which associates with the clinical stage, disease progress and prognosis.

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