Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (10): 1803-1808.doi: 10.3969/j.issn.1673-8225.2011.10.020

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Reconstruction of hepatic functions after bone marrow mesenchymal stem cells transplantation

Liu Fang1,2, Chen Shao-hong2, Liu Qing-bo2, Fan Xin-juan2, Tang Fang2, Zhao Guo-qiang2   

  1. 1Department of Pathology, University of South China Medical College; University Key Laboratory of Cancer Cellular and Molecular Pathology in Hunan province, Hengyang   421001, Hunan Province, China
    2Department of Pathology, Zhongshan School of Medicine, Sun Yat-sen University;  Department of Pathology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou    510080, Guangdong Province, China
  • Received:2010-09-27 Revised:2010-11-11 Online:2011-03-05 Published:2011-03-05
  • Contact: Zhao Guo-qiang, Doctor, Professor, Department of Pathology, Zhongshan School of Medicine, Sun Yat-sen University; Department of Pathology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • About author:Liu Fang★, Master, Lecturer, Department of Pathology,University of South China Medical College; University Key Laboratory of Cancer Cellular and Molecular Pathology in Hunan province, Hengyang 421001, Hunan Province, China; Department of Pathology, Zhongshan School of Medicine, Sun Yat-sen University; Department of Pathology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080,Guangdong Province,China lf5527@163.com
  • Supported by:

    the National Natural Science Foundation of China, No.30170473*; the Natural Science Foundation of Guangdong Province, No.04009326*; the Research Foundation of Education Bureau of Hunan Province, No. 08C744*; the Research Foundation of University Key Laboratory in Hunan Province, No. 09K074*, 09K075*

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Abstract:

BACKGROUND: Cell transplantation is an effective method for the treatment of hepatic function failure. However, ideal cell source and the best way to transplant has been the target of the ongoing research in laboratories.
OBJECTIVE: To observe the effect and optimal transplantation way of bone marrow mesenchymal stem cells (BMSCs) transplantation on hepatic function reconstruction with acute hepatic failure in rats.
METHODS: D-galactosamine (D-gal) was served as hepatic toxic agent, female SD rats with acute hepatic failure model was constructed. Male rats BMSCs as cell source was implanted hepatic failure rats through groin vein and intrasplenic transplantation. Many hepatic function blood biochemical indices were detected dynamically. Sry gene was served as molecule marker, the survival status of BMSCs in hepatic was detected by PCR technique.
RESULTS AND CONCLUSION: BMSCs through groin vein and intrasplenic transplantation can lower the levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBIL), improve the ability of albumin (ALB) synthesis, ameliorate hepatic function, and reduce the death rate of acute hepatic failure rats. The death rate in the group of groin venous transplantation was lower than that in the intraspleen transplantation group. Sry gene positive cell was detected in hepatic of receptor rats at 7-30 days after transplantation; however, sry gene positive cell was not detected in hepatic of receptor rats in corresponding control group. The results showed that BMSCs can ameliorate hepatic function of acute hepatic failure rats. BMSCs can survive in the hepatic of receptor rats. The operation of groin venous transplantation is simple and convenient; the death rate is lower. So groin venous transplantation is a better way to treat acute hepatic failure rats.

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