Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (10): 1773-1776.doi: 10.3969/j.issn.1673-8225.2011.10.014

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Auxiliary role of origin recognition complex binding site for silencer involved in transcriptional silencing

Zhang Xin-min1,2, Wang Hao-tian1, Liu Nan1, Yu Qun2, Yan Wei-qun1, Bi Xin2   

  1. 1School of Pharmaceutical Sciences, Jilin University, Changchun  130021, Jilin Province, China; 2Department of Biology, University of Rochester, Rochester, NY  14627, USA
  • Received:2010-12-01 Revised:2011-01-25 Online:2011-03-05 Published:2011-03-05
  • Contact: Yan Wei-qun, Professor, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, Jilin Province, China weiqunyanjlu@126.com
  • About author:Zhang Xin-min☆, Studying for doctorate, School of Pharmaceutical Sciences, Jilin University, Changchun 130021, Jilin Province, China xmzhangjlu@126.com

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Abstract:

BACKGROUND: Silencer is consisted of origin recognition complex (ORC), RAP1 and other elements. There were a lot of reports on function and relationship between silencers but few researches concerning silencer elements.
OBJECTIVE: To study the auxiliary gene silencing effect of ORC to silencer.
METHODS: Delete or replace HML-I silencer with ORC binding site with URA3 in the right side or blank sequence without any binding sites to compare expression level of URA3 and analyze the difference of chromatin structure with DNA topological method.
RESULTS AND CONCLUSION: When HML-E silencer exited alone, yeast could not grow on FOA plates. But with auxiliary effect of ORC binding site, it was able to grow on the FOA plate, and the proportion of negative super coiled DNA was larger compared with that in the case that HML-E silencer exits alone. Therefore, ORC can interact with HML-E to condense the chromatin structure and enhance gene silencing.

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