Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (10): 1745-1749.doi: 10.3969/j.issn.1673-8225.2011.10.008

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Human umbilical cord mesenchymal stem cells differentiate into Schwann cells in vitro

Wang Guang-yu, Zhao Fang, Hou Rong-rong, Zhu Lü-yun, Ma Li-cheng, Hu Li-ye, Li Xiao-ling, Yang Shao-ling, Shan Wei   

  1. Department of Endocrinology, Bethune International Peace Hospital of Chinese PLA, Shijiazhuang  050082, Hebei Province, China
  • Received:2010-10-10 Revised:2010-11-18 Online:2011-03-05 Published:2011-03-05
  • About author:Wang Guang-yun★, Master, Associate chief physician, Department of Endocrinology, Bethune International Peace Hospital of Chinese PLA, Shijiazhuang 050082, Hebei Province, China yangzy_1220@yahoo.com.cn
  • Supported by:

    the Key Program of Medical Scientific Research of Hebei Province in 2007, No. 07438*

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Abstract:

BACKGROUND: Human umbilical cord Wharton's Jelly mesenchymal stem cells avoid the ethical constraints, which have rich resources, and can be used as seed cells for tissue repair.
OBJECTIVE: To observe the feasibility of umbilical cord Wharton's Jelly mesenchymal stem cells (UC-MSCs) differentiated into Schwann cells in vitro.
METHODS: UC-MSCs were isolated and cultivated. The surface markers of UC-MSCs were identified by flow cytometry. UC-MSCs were induced and differentiated into Schwann cells by two stages with neurons medium, basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), retinoic acid (RA), platelet-derived growth factor (PDGF). Morphologic changes of cells were observed under inverted microscope. The expression of Nestin, S-100, and glial fibrillary acidic protein (GFAP) were detected by immunocytochemical stain method. Specific proteinum expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western Blot technique.
RESULTS AND CONCLUSION: Morphous of UC-MSCs were changed at 7 days culture. A part of UC-MSCs changed into fusiform shape. The primary cell could achieve 80%–90% confluence about 10 days, the morphous of UC-MSCs were fusiform shape. Cells isolated from human umbilical cord expressed specific mesenchymal stem cells surface markers: CD44 (91.4%), CD29 (91.3%), CD105 (99.2%); without expression CD34 (0.2%), CD45 (0.9%), CD14 (0.6%). Morphous of UC-MSCs changed from short fusiform shape to long fusiform shape or fusiform, cells assembled and some global cell groups formed after the first induction stage. After the second induction stage, some long fusiform shape cells grown from global cell groups. Morphous of major cells were long fusiform shape after 96 hours with the phenomenon of multipolar. The immunocytochemical stain showed that long fusiform cells have Schwann cells specific GFAP and S100 protein staining. The results showed that UC-MSCs could be differentiated into Schwann cells in vitro.

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