Abstract:

BACKGROUND: In order to preserve the greatest bioactivity of the tendon, a kind of allograft preserving liquid is needed.
OBJECTIVE: To observe the cell morphology and tendon bioactivity at different time points, followed by protection of the rabbit tendon in pure glycerine under normal temperature.
METHODS: After special procession, the rabbit tendons were tightly sealed in pure glycerine away from light under normal temperature. The organizational structure and cell morphology of tendon were observed under electron microscope with hemotoxylin and eosin (HE) staining for paraffin section, and the bioactivity was observed by superoxide dismutase zymetology experiment for 2, 4, 7 and 12 months, respectively.
RESULTS AND CONCLUSION: HE stained paraffin section showed that in 2, 4, 7 and 12-month glycerine group, most cell membranes were intact, nucleuses inerratic, and tendon organizational structures existed. The electron microscope showed the cells were in static, and the nucleus patterns were normal. The superoxide dismutase zymetology experiment showed that the tendon owned the superoxide dismutase enzymatic activity. The results indicated that the organizational structure, most of cell morphology and bioactivity of the tendon were remained, following the tendon was specially processed and tightly sealed in pure glycerine away from light under normal temperature for 12 months..