Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (53): 9968-9972.doi: 10.3969/j.issn.1673-8225.2010.53.022

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Intrahepatic islet transplantation through the portal vein in rats

Yang Shun-liang1, Wu Zhi-xian1, Ye Yong-feng2, Cai Jin-quan1, Wang Qing-hua1, Huang Liang-hu1, Lin Guo-zhang 1, Zhou Hao1,  Tan Jian-ming1   

  1. 1 Department of Urinary Surgery, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou   350025, Fujian Province, China; 2 Department of Urinary Surgery, the 184 Hospital of Chinese PLA, Yingtan   335000, Jiangxi Province, China
  • Online:2010-12-31 Published:2010-12-31
  • Contact: Tan Jian-ming, Chief physician, Professor, Department of Urinary Surgery, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou 350025, Fujian Province, China
  • About author:Yang Shun-liang★, Master, Associate chief physician, Department of Urinary Surgery, Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA, Fuzhou 350025, Fujian Province, China ysliang@medmail.com.cn
  • Supported by:

    the Medical Science and Technology Innovation Foundation of Nanjing Military Area Command of Chinese PLA in 2007*; the Major Program of Science and Technology Plan of Fujian Province, No. 2009Y4001*; the Science and Technology Innovation Platform Plan Program of Fujian Province, No. 2008J0106*

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Abstract:

BACKGROUND: The liver is commonly used ideal site because the liver is not only the site of insulin action, but also a relative immunologically privileged site. Furthermore, the volume of liver is big enough for transplantation; the construction of sinus hepaticus and vein is profited for dwell and growth of islet cell.
OBJECTIVE: To investigate the method of intrahepatic islet transplantation through portal vein in Sprague Dawley rats with type I diabetes.
METHODS: Islet cells of Sprague Dawley rats were prepared by methods of a previous study. Type I diabetes mellitus was induced with streptozotocin in Sprague Dawley rats via intraperitoneal injection. The rats were divided into two groups. 1 000 IEQ islets (1.5 mL) were infused into the liver of Sprague Dawley rats by the main portal vein puncture as experimental group, and serum-free 1640 medium (1.5 mL) was infused as control group. No immunosuppressive drug was administered after operation. Bleeding volume, blood glucose, insulin level and histomorphological changes in the liver were observed.
RESULTS AND CONCLUSION: All rats survived. Success rate of portal vein puncture was 90% with bleeding volume less than 0.5 mL. Duration of normal blood glucose after operation in experimental group was 1 to 6 days (3.7 ± 1.7) days. The islet graft survival duration was 2 to 15 days (8.4 ± 4.1) days. Serum insulin levels in experimental group were significantly higher compared with control group within 2 weeks post-operative (P < 0.05, P < 0.01). The pathologic examination has confirmed that the morphous of hepatocyte and structure of hepatic lobule were normal. There was no necrosis or infection lesion in liver parenchyma and thrombopoiesis. The stenosis of main portal vein did not occur. Islet graft was pathologically viable and functioning in hepatic sinusoids. These have suggested that intrahepatic islet transplantation through the main portal vein in rats is an acceptable method.

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