Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (53): 9946-9949.doi: 10.3969/j.issn.1673-8225.2010.53.016

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Intrathymic injection of allogenic antigen induces allograft immune tolerance in rats

Yue Wei-jie1, Cui Yong1, Li Wei1, Yue Lei1, Xie Gang2, Meng Qing-gang1   

  1. 1 Department of Orthopaedics, Fourth Affiliated Hospital of Harbin Medical University, Harbin  150001, Heilongjiang Province, China; 2 Department of Orthopaedics, Harbin Fourth Hospital, Harbin  150001, Heilongjiang Province, China
  • Online:2010-12-31 Published:2010-12-31
  • Contact: Meng Qing-gang, Professor, Doctoral supervisor, Department of Orthopaedics, Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China mengqinggang2009@126.com
  • About author:Yue Wei-jie★, Master, Chief physician, Professor, Department of Orthopaedics, Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China jbjsbr@126.com
  • Supported by:

    Medical Scientific Research of Health Department of Heilongjiang Province, No. 2009-178*, 2009-174*; Supporting Item of Education Department of Heilongjiang Province, No. 11541147*; the Tackle Key Program in Science and Technology of Heilongjiang Province, No.GC10C303-4*

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Abstract:

BACKGROUND: Similar to other foreign tissues, allogeneic bones also have antigenicity, which can result in rejection following transplantation. Though the application of immunosuppressant can suppress immunological rejection, it has certain harmful effects to the body.
OBJECTIVE: To investigate the effects of intrathymic injection of allogenic antigen on allograft immune tolerance in rats.
METHODS: Totally 60 Wistar rats were prepared for bone defects models and randomly divided into allogeneic bone graft, autogenous bone graft + allo-antigen group and allogeneic bone graft + immunosuppressant groups. Prior to transplantation, rats in the allogeneic bone graft + allo-antigen group were received intrathymic injections of spleen cells extracted MHC antigens. X-ray examination, hematoxylin-eosin staining were used to observe bone healing at 1, 2, 4 and 6 weeks after transplantation. Meanwhile, the soluble interleukin-2 receptor and mixed lymphocyte were tested by immunological assays.
RESULTS AND CONCLUSION: Gross observation, X-ray film and histological examination showed that there were inflammatory cell infiltration and new bone formation in the allogeneic bone graft + allo-antigen and autogenous bone graft groups. In the allogeneic bone graft + immunosuppressant group, the regenerated vessel was few, and the bone healing was delayed, the inflammatory cell infiltrate was notable, and no new bone was formed. It confirmed that intrathymic injections of spleen cells extracted MHC antigens can induce recipient tolerance to donors bone graft. Immunologic test showed that the bone formation in the allogeneic bone graft + allo-antigen group was similar to the autogenous bone graft group, which was better than the allogeneic bone graft + immunosuppressant group. Thus, intrathymic injection of allo-antigen can induced on the allograft immune tolerance.

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