Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (53): 9941-9945.doi: 10.3969/j.issn.1673-8225.2010.53.015

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Anti-injury treatment effects on transplant outcomes following cryopreserved ovarian tissue transplantation

Zhang Shuang, Wang Dong, Jiang Ying-yan, Ren Bao-hong, Duan Chun-feng   

  1. Department of Gynaecology and Obstetrics, the 465 Hospital Affiliated to Jilin Medical College, Jilin  132013, Jilin Province, China
  • Online:2010-12-31 Published:2010-12-31
  • Contact: Wang Dong, Master’s supervisor, Professor, Chief physician, Department of Gynaecology and Obstetrics, the 465 Hospital Affiliated to Jilin Medical College, Jilin 132013, Jilin Province, China wangdong1960@126.com
  • About author:Zhang Shuang★, Studying for master’s degree, Physician, Department of Gynaecology and Obstetrics, the 465 Hospital Affiliated to Jilin Medical College, Jilin 132013, Jilin Province, China ciea_liu@126.com
  • Supported by:

     the Science and Technology Development Project of Jilin Province, No. 200705415*

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Abstract:

BACKGROUND: Transplantation of ovarian tissue is performed without vascular reanastomosis. To ameliorate the tolerance of ovarian tissue to injuries is critical for follicular survival and for exerting functions of the cryopreserved graft.
OBJECTIVE: To compare the effects of vascular endothelial growth factor (VEGF), vitamin E, milkvetch root anti-injury treatment on morphology and function of freeze ovarian tissue after cryopreserved ovarian tissue autologous heterotopic transplantation.
METHODS: Adult female Wistar rats were randomly assigned to four groups: normal control group, ovariectomy group, autologous fresh ovarian tissue transplantation group (non-cryopreserved ovarian tissue, direct transplantation) and cryopreserved ovarian tissue transplantation group. The cryopreserved ovarian tissue transplantation group was divided into three subgroups: non-anti-injury treatment group, VEGF + vitamin E + milkvetch root group and VEGF + vitamin E anti-injury group. At 2 months posttransplantation, serum estradiol (E2) level was detected. Following hematoxylin-eosin staining, follicular morphology and number of normal ovarian follicle were observed.
RESULTS AND CONCLUSION: E2 levels were increased in the VEGF + vitamin E + milkvetch root group compared with no drug group, and number of primary follicle was increased (P < 0.05). The anti-injury drug affected ovary shape and functional recovery. E2 levels and number of primary follicle were greater in the VEGF + vitamin E + milkvetch root group compared with VEGF + vitamin E anti-injury group, but no significant difference was found. We could not confirm that the milkvetch root anti-injury treatment obtained good outcomes. These indicate that VEGF + vitamin E + milkvetch root anti-injury treatment exerts effects on the recovery of ovary shape and function following autologous heterotopic transplantation.

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