Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (53): 9933-9936.doi: 10.3969/j.issn.1673-8225.2010.53.013

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Establishment of left kidney transplantation models in rats with bilateral kidney grafts and monitoring of kidney functions

Fang Cheng1, Liu Li1, Hu Han-ning2, Chen wei2, Wang Jia-mou1, Zhang Qi-feng1, Qu Yong-hua1   

  1. 1 Laboratory of Transplantation Engineering, Wuhan Polytechnical University, Wuhan  430023, Hubei Province, China; 2 Department of Clinical Laboratory, Zhongnan Hospital, Wuhan University, Wuhan  430071, Hubei Province, China
  • Online:2010-12-31 Published:2010-12-31
  • About author:Fang Cheng☆, Doctor, Associate professor, Laboratory of Transplantation Engineering, Wuhan Polytechnical University, Wuhan 430023, Hubei Province, China fang_cheng@yahoo.cn
  • Supported by:

    the Natural Science Foundation of Hubei Province, No. 2008CDB150*

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Abstract:

BACKGROUND: Kidney transplantation in rats is used as an important experimental model for researches of immune tolerance, immune suppression and orange resources.
OBJECTIVE: To develop the kidney transplant models of tolerance, acute and chronic rejection, to investigate surgical technical modifications, and to develop a non-damaging examination of function of the kidney graft.
METHODS: Orthotopic kidney transplantation was performed with end to end anastomosis. The left side kidney of the recipient was replaced by a donor allograft and a contralateral nephrectomy was performed 7 days later. The left and right kidney of the donor could be used apart in-order. Lewis recipients with a DA allograft served as acute rejection group. Lewis recipients with a F344 allograft served as chronic rejection group. Lewis rats of auto-transplantation served as immune tolerance groups. The application anatomies of bilateral kidney, the warm ischemia time and cold ischemia duration of graft, the success ratio of transplantation, as well as the survival curve and proteinuria changes were observed.
RESULTS AND CONCLUSION: A total of 43 rats were anatomized, 29 kidneys were irrigated, and 21 grafts were transplantation. The warm ischemia duration less than 10 seconds, the cold ischemia duration was (47.2±3.7) minutes, and the success ratio of transplantation was 85.7% (18/21), including 3 rats in the autotransplantation group, 3 in the acute rejection group and 12 in the immune tolerance group. The present operation is simplified, stable, and economical. It avoids impairment of the abdomen aorta and the inferior vena cava of recipients．The survival curve and proteinuria trend showed significant differences between acute rejection, chronic rejection and immune tolerance groups.

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