Abstract:

BACKGROUND: Liver acute rejection is one of the most severe complications that may blast the graft. Now liver biopsy is the main way to diagnose it, which may bring about some complications such as infections and hemorrhages. So it is one of the most important focal points in the field of liver transplantation to discover a non-invasion or less-invasion index to diagnosis and forecast of liver acute rejection.
OBJECTIVE: To explore the relationship of the expression of interleukin-2, interleukin-10, human leukocyte antigen-G (HLA-G) and acute rejection in liver transplantation.
METHODS: Fifty-nine blood samples were taken from cases after liver transplantation. The expression of interleukin-2, interleukin-10 and HLA-G was assayed by ELISA. The cases were divided into the acute rejection group and non-rejection group, and the latter group was divided into normal liver function group and abnormal group. The results were analyzed and ROC curve was made. Area under ROC curve and the sensibility and specificity of the cut-off point were analyzed statistically. 
RESULT AND CONCLUSION: The expressions of interleukin-10 and HLA-G in serum of the acute rejection group was obviously lower than those in the non-rejection group (P=0.032, 0.002), while expression of interleukin-2 in acute rejection group was higher than that in the non-rejection group (P=0.002). The expression of interleukin-2 was statistically higher in the abnormal liver function group than that in the normal liver function group (P=0.02), while the expression of interleukin-10 and HLA-G had no statistical difference in the two groups (P=0.525, 0.084). The expression of interleukin-2, interleukin-10 and HLA-G is connected with liver acute rejection. And in the three factors HLA-G is the most effective index for diagnosis and forecast of liver acute rejection.