Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (53): 9913-9916.doi: 10.3969/j.issn.1673-8225.2010.53.008

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Influences of hepatic artery/portal vein early reflow on rat intestinal ischemia/reperfusion injury following liver transplantation

Zhang Pei-jian, Zhuang Zhuo-nan, Tian Ming-xiang, Su Hui, Bu Ping, Meng Zhong-liang, Zhang Jie, Wu Xue-yan   

  1. Department of General Surgery, Yangzhou University School of Medicine, Yangzhou  225001, Jiangsu Province, China
  • Online:2010-12-31 Published:2010-12-31
  • Contact: Bu Ping, Doctor, Professor, Chief physician, Doctoral supervisor, Department of General Surgery, Yangzhou University School of Medicine, Yangzhou 225001, Jiangsu Province, China boping@yzu.edu.cn
  • About author:Zhang Pei-jian★, Master, Professor, Chief physician, Master’s supervisor, Department of General Surgery, Yangzhou University School of Medicine, Yangzhou 225001, Jiangsu Province, China yzu.edu.pjz@163.com
  • Supported by:

     the Science Research Foundation of Health Department of Jiangsu Province, No. H200770*; Item for Traditional Chinese Medicine in Jiangsu Province, No. LZ09133*; the Tackle Key Program in Science and Technology of Yangzhou City, No. YZ2008042*

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Abstract:

BACKGROUND: It remains unclear that whether different time sequence of hepatic artery/portal vein early reflow can aggravate the intestinal ischemia/reperfusion (I/R) injury following liver transplantation.
OBJECTIVE: To investigate the influence of hepatic artery/portal vein early reflow on rat small bowel I/R injury after liver transplantation.
METHODS: Rat liver transplantation models with portal vein infusion were selected. A total of 78 SD rats were randomly divided into the 3 groups. The hepatic artery group (n=36): rats were underwent liver transplantation, and received infusion via portal vein using 40 C Ringer lactate solution, opened hepatic artery and inferior vena cava, followed by open portal vein 10 minutes later; the portal vein group: rats were underwent liver transplantation, open the hepatic artery at 10 minutes after portal vein; the sham-surgery group received abdominal cavity exposure and liver liberation. The microstructure and ultramicrostructure changes of the small bowels were observed, and the level of the nitric oxide (NO) was determined.
RESULTS AND CONCLUSION: In each group at different periods after operation, the villi had occurred malposition or disorder. The mitochondria size of mucous membrane of small intestine cell were not the same, and engorged obviously, were almost buninoid, vacuolar degeneration were inside. The cristae of mitochondria were decreased, collapsed or disappeared. The NO level of small intestine tissue was raised. These changes reached the peak at 12 hours after operation. Compared with the portal vein group, the injury of microstructure and ultramicrostructure of small bowel and NO level of small intestine tissue of the hepatic artery group were higher. Through early oxygen supply of liver, hepatic artery early reflow can reduce transplantation liver damage, the delay in opening the portal vein increased small intestine of I/R injury of liver transplantation in rats.

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