Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (53): 9888-9892.doi: 10.3969/j.issn.1673-8225.2010.53.002

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Expression of peroxisome proliferator activated receptor gamma in obliterative bronchiolitis following lung transplantation

Liu Hong-xu1, Wang Ge-bang1, Liu Yang2, Xu Shun1, Zhang Lin1   

  1. 1 Department of Thoracic Surgery, First Affiliated Hospital, 2 Deparment of Pathology, Basic Medical College, China Medical University, Shenyang  110001, Liaoning Province, China
  • Online:2010-12-31 Published:2010-12-31
  • About author:Liu Hong-xu☆, Doctor, Professor, Doctoral supervisor, Department of Thoracic Surgery, First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, China hongxuliu@yahoo.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30700821*

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Abstract:

BACKGROUND: Peroxisome proliferator activated receptor γ (PPAR γ) plays some roles in inhibiting tissue fibrosis and modulating immune activity. However, little is known about its function in solid organ transplantations. It might be an ideal factor for the inhibition of rejection response.
OBJECTIVE: In order to investigate the mechanism of PPAR γ, we detected its expression in obliterative bronchiolitis after pulmonary transplantation.
METHODS: After ectopic tracheal transplantation in mice, immunohistochemistry staining was employed to detect the location of PPAR γ in different kinds of cells at different stages of post-transplantation animals. The expression of PPAR γ mRNA in transplanted trachea was analyzed with RT-PCR. Airway fibroblasts was primarily cultured and treated with transforming growth factor-β1 (TGF-β1) at 5 mg/L. At different time points, the expression of PPAR γ mRNA in fibroblasts was analyzed with RT-PCR. The expression of α-smooth muscle antigen-the marker of myofibroblasts was examined with Western blot after the fibroblasts were treated with TGFβ-1 (5 mg/L) or PPAR γ ligand-Rosiglitazone (10 mmol/L) at specific time interval. The expression of α-smooth muscle antigen in cultured pulmonary fibroblasts was investigated by Western Blot.
RESULTS AND CONCLUSION: In the inflammatory stage of post-transplantation, PPAR γ was found in the inflammatory cells, while in the fibroblasts in the fibrous proliferation stage. Markedly dropped of mRNA of PPAR γ was detected in the tissue homogenate of transplanted trachea. In the same way, the amount of PPAR γ mRNA remarkably diminished after the cultivated pulmonary fibroblasts were treated with TGF-β1. The addition of PPAR γ ligand-rosiglitazone could inhibit the production of α-smooth muscle antigen induced with TGF-β1. The results demonstrated that decreased expression of PPAR γ could be one of the most important factors contributing to the development of obliterative bronchiolitis after pulmonary transplantation. The activation of PPARγ could inhibit the differentiation of myofibroblasts induced by TGF-β1.

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