Abstract:

BACKGROUND: Clinical application of low potassium dextran (LPD) solution has better effectiveness, but effective period of donor lung preservation is still short and price is expensive.
OBJECTIVE: To observe changes of lung preservation after different periods preserved with modified self-confected LPD solution and to evaluate the storage term of lung in this solution.
METHODS: In total 32 healthy dogs were randomly divided into the control group and experimental group. Dogs in the control group were perfused with LPD solution; and those in the experimental group were perfused with modified self-confected LPD solution. Changes of myeloperoxidase, malonaldehyde, wet to dry weight ratio (W/D), pathological result and super-micro structural results from donor lung tissues were compared at pre-perfusion, after perfusion, and 4-, 8-, 12-, as well as 24-hours of preservation.
RESULTS AND CONCLUSION: For both groups: the values of myeloperoxidase, malonaldehyde and W/D were increasing as lung preservative period in vivo was prolonging; after 12-hour preservation, the value of myeloperoxidase was significant changed while the value of malonaldehyde and W/D were changed significantly after 8-hour preservation; the pathological and super-micro structural changes were reversible till lung preservative period reached 8-hour. Changes of lung preservation started to be significant after 8-hour preserved with modified self-confected LPD solution and irreversible historical changes appeared after 12-hour preservation.