Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (44): 8352-8356.doi: 10.3969/j.issn.1673-8225.2010.44.045

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Donor antigenic specificity CD4+CD25+Treg cells prolong the survival of allograft kidney in rats

Li Jian1, Xu Ya-hong1, Ma Xiao-ping1, Gu Xin-wei1, Zhang Gen-fu2, Huang Chi-bing2   

  1. 1 Department of Urology, the 452 Hospital of Chinese PLA, Chengdu 610061, Sichuan Province, China; 2 Department of Urology , Xinqiao Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China
  • Online:2010-10-29 Published:2010-10-29
  • Contact: Huang Chi-bing, Doctor, Professor, Master’s supervisor, Department of Urology , Xinqiao Hospital, Third Military Medical University of Chinese PLA, Chongqing 400038, China
  • About author:Li Jian★, Master, Attending physician, Department of Urology, the 452 Hospital of Chinese PLA, Chengdu 610061, Sichuan Province, China apollo99101@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30571863*

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Abstract:

BACKGROUND: the development of magnetic separation technique, it is feasibility to in vitro sort and amplify CD4+CD25+Treg cells for transplantation; however, the application dosage and immune tolerance have been less reported yet.
OBJECTIVE: To investigate dose-effect relationship of CD4+CD25+Treg cells during allograft transplantation.
METHODS: SD rats which were considered as the donors and Wistar rats as receptors were used to establish allograft kidney transplantation models. CD4+CD25+Treg cells were separated from splenic cells of Wistar rats and induced phenotype of donor antigenic specificity in vitro. According to the quantities of CD4+CD25+Treg cells injecting through tail vein during the operation of allograft kidney transplantation, models were rolled into four experiment groups: group 1 (2×105), group 2 (5×105), group 3 (1×106), and group 4 (2×106). The models out injection were considered as controls. Survival status of kidney was detected at day 15 postoperatively; creatinine level and pathological changes were detected at days 4, 9 and 15 according to Banff Schema diagnostic standard; semi-quantitative scores were measured Watanabe technique.
RESULTS AND CONCLUSION: The death rate was the highest in control group (83.3%), and then group 1 (66.7%), group 4 (58.3%), and group 2 (33.3%); but rats in the group 3 were all survival. Creatinine level in experimental groups was significantly less than control group at days 4, 9, and 15 postoperatively (P < 0.05, P < 0.01); the creatinine levels in the group 1 and group 2 were significantly greater than in the group 3 and group 4 at days 9 and 15 postoperatively (P < 0.05). Semi-quantitative scores demonstrated that there was no significant difference between group 2 and group 1; but the scores in the group 3 and group 4 were significantly greater than control group (P < 0.05). The results indicated that CD4+CD25+Treg cells could improve kidney function following transplantation, and prolong survival time of transplanted kidney. The 1×106 was the best dosage for application.

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