Abstract:

BACKGROUND: Currently, rejection is inhibited by blocking T cell activation. Many in vitro experiments showed that, astilbin and their analogs exhibit typical selective immunosuppression.   
OBJECTIVES: To investigate the expression and implication of astilbin on peripheral blood T cell activation in rats with lung transplantation.
METHODS: Fifty rats were randomly divided into 3 groups. Ten rats were taking peripheral blood as the blank group. The remained 40 rats were randomly assigned to donors and recipients. Model of rat left lung transplantation was set up. Twenty lung transplanted rats were divided in to 2 groups randomly: the control group, which was lavaged with normal saline, the experimental group was lavaged with astilbin. The expressions of T cell activation antigen CD69, CD25 and CD71 were determined by fluorescent-labeled antibody staining and flow cytometry (FCM) prior to and at 2, 5 days after transplantation.
RESULTS AND CONCLUSION: The levels of CD69, CD25 and CD71 in the astilbin group were markedly higher at 2 days after lung transplantation than that of the blank group (P < 0.01), but lower than the control group (P < 0.05). At 5 days after lung transplantation, the levels of CD69, CD25 and CD71 in the astilbin group were declined into normal levels, which was notably lower than that of the control group (P < 0.01). Astilbin significant suppresses acute rejection-induced T cell activation and shows strong inhibition effect on rejective reaction after lung transplantation.