Abstract:

BACKGROUND: Previous research has demonstrated that in vitro pre-treatment and conservation of blood vessels were improved remarkably; however, they remained still satisfactory. Therefore, to look for effective method still needs to be further studied.
OBJECTIVE: To compare vitrification and traditional freezing to preserve the allograft vascular effects after transplantation, and to find a more practical and easy production of vascular treatment methods.
METHODS: A total of 96 health bruising-blue rabbits, undergoing surgical resection of bilateral femoral artery, were divided into 3 groups: fresh vascular autograft (group A), irradiation pretreatment frozen vascular allograft (group B), and vitrification + irradiation pretreatment of vascular allograft (group C), according to different methods of in vitro vascular preconditioning experiment. At 1, 4, 8, and 12 weeks after transplantation, six experimental animals were taken from each group for abdominal aortic digital subtraction angiography, scanning electron microscopy, histopathology and other methods to observe the rate of aneurysm formation, morphological changes and organization of the vascular graft.
RESULTS AND CONCLUSION: At 12 weeks after transplantation, the cumulative patency rate in the group A was significantly higher than that in the group B (P < 0.05); however, there was no significant difference between group C and group A as well as group B (P > 0.05). Histopathological examination revealed that allograft vascular smooth muscle proliferation in endometrium and middle layer of group C was slightly compared with group B, showing unclear lumen stenosis and light inflammatory reaction. The vitrification irradiation preservation of allograft blood vessels, making the procedure easy, high patency rate of grafts, tissue reaction to light, was an ideal way to deal with allogenic blood vessels in vitro.