Abstract:

BACKGROUND: It is the foundation and prerequisite for relative experimental research that whether reduce-size liver transplantation in rat was established successfully. Respiratory arrest is the main cause effecting on rat model of reduce-size liver transplantation. It is important to rise succeed rate of rat-reduce-size liver transplantation by analyzing cause and emergency treatment of intra-operation respiratory arrest. 
OBJECTIVE: To analyze cause of respiratory arrest during reduce-size liver transplantation of the rat and to investigate emergency treatments for respiratory arrest. 
METHODS: Healthy SD rats were selected. Female rats served as donors and the male rats served as recipients. Operation of donor was performed by only one person with the naked eye, which reduced-size donor liver was performed in the donor operation. Operation of the receptor was performed by two persons with the naked eye, with improved dual-cuff technique of Kamada and stay pipe of biliary tract. The recipients with respiratory arrest were divided into 3 groups as follows: Experimental group 1: the recipients with respiratory arrest accepted “simple equipped artificial respiration” and external chest compression; Experiment group 2: the recipients with respiratory arrest only accepted “simple equipped artificial respiration”; Control group: the recipients with respiratory arrest only accepted external chest compression. 
RESULTS AND CONCLUSION: A total of 270 rats were accepted reduce-size liver transplantation successfully, 50 of 270 rats underwent intra-operative respiratory arrest, the cause of intra-operative respiratory arrest as follows: 42 rats showed anesthesia overdose, 3 rats showed excessive gas embolism, 3 rats showed pneumothorax and 2 rats showed acute pulmonary edema. The success rates of emergency treatment for respiratory arrest in the experimental group 1, experimental group 2, and the control group were: 84.6% (22/26), 54.5% (6/11), and 30.8% (4/13), respectively. The success rate of emergency treatment for anesthesia overdose-induced respiratory arrest in the experimental group 1, experimental group 2, and the control group were was 95.6% (22/23), 66.7﹪(6/9), and 40% (4/10), respectively. The most common respiratory arrest cause during reduce-size liver transplantation of rat was anesthesia overdose, then excessive gas embolism, pneumothorax and acute pulmonary edema in proper order. The most effective emergency treatment of respiratory arrest of rats was “simple equipped artificial respiration” and external chest compression, then “simple equipped artificial respiration” and external chest compression in proper order.