Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (44): 8183-8185.doi: 10.3969/j.issn.1673-8225.2010.44.004

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Establishment of a rat orthotopic left lung transplantation model using modified three-cuff method

Fan Kai, Wang Jian-jun, Zhai Wei, Tang Jian, Wan Li   

  1. Department of Thoracic Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan  430022, Hubei Province, China
  • Online:2010-10-29 Published:2010-10-29
  • Contact: Zhai Wei, Doctor, Associate Chief physician, Associate professor, Department of Thoracic Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
  • About author:Fan Kai★, Studying for master’s degree, Department of Thoracic Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China fankay0922@gmail.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30700767*

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Abstract:

BACKGROUND: Lung transplantation is the sole cure for end-stage lung disease. The basic research addressing lung transplantation is based on animal models.
OBJECTIVE: To establish a new simple, stable, and available experimental model of rat lung orthotopic allograft transplantation.
METHODS: Fifty male rats were randomly grouped into donors and recipients; lung transplantation was performed with using new three-cuff technique. The donor lung was harvested, placed over left lung of recipients and maintained in 4℃ LPD solution. The donor lung was anastomosed to the recipient following the sequence from the left pulmonary artery, vein to the bronchus. Then the left pulmonary vein-artery was opened successively, and the left lung of recipient was removed. Blood gas of recipients was tested after 5 weeks.
RESULTS AND CONCLUSION: The successful rate of surgery was 92%, and the mean time for all the proceedings was (55±3) minutes. All the lung grafts were survivable over 5 weeks. Blood was collected from the left vein at 15 minutes prior to and after right pulmonary hilum occlusion, and the blood gas analysis showed there was no significant difference of the blood gas before and after occlusion (P > 0.05). The findings suggested that, new cuff method is a simple, reproducible and valid method for establishing models of rat orthotopic left lung transplantation.

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