Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (44): 8171-8174.doi: 10.3969/j.issn.1673-8225.2010.44.001

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Inhibition effect of p53 gene on coronary artery intima hyperplasia following heart transplantation

Wang Li-ping1, Liu Yue2, Jia Zhi-bo3, Li Ying1, Zhang Yi-na1, Yin Xin-hua2   

  1. 1 Department of Senile Disease, 3 Department of Cardiac Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin  150086, Heilongjiang Province, China; 2 Department of Cardiology, the First Affiliated Hospital of Harbin Medical University, Harbin  150001, Heilongjiang Province, China
  • Online:2010-10-29 Published:2010-10-29
  • Contact: Jia Zhi-bo, Associate chief physician, Department of Cardiology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
  • About author:Wang Li-ping☆, Doctor, Attending physician, Department of Senile Disease, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China wlp0502@yahoo.com.cn
  • Supported by:

    the Scientific Research Foundation for Doctors of the Second Affiliated Hospital of Harbin Medical University, No. BS2008-25*; the Science and Technology Research Foundation of Education Department of Heilongjiang Province in 2010, No.11551256*

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Abstract:

BACKGROUND: Wild-type p53 gene can inhibit vascular smooth muscle cells proliferation, relieve graft coronary artery vascular intima hyperplasia and lumina narrow after vascular intima injury. 
OBJECTIVE: To evaluate the inhibition effect of wild-type p53 gene transfer on graft coronary artery intima hyperplasia after heart transplantation.
METHODS: Rat model of heterotopic (abdomen) heart transplantation was developed. Wistar rats served as donors and SD rats as recipients. After donor hearts were removed, 800 µL adenoviral vector encoding the wild-type p53 gene (Ad-p53 group), adenoviral vector encoding the β-galactosidase gene (LacZ) (Ad-LacZ group) or saline (control group) were infused into the donor heart respectively before transplantation. The donor heart was stored in the 4 ℃ saline for 30 minutes before heart transplantation. p53 gene mRNA expressions in coronary artery of donor hearts were tested by reverse transcription PCR at 5 days after operation. At 28 days after transplantation, the donor hearts and coronary artery were observed under a microscope, and the ratio of intima/media thickness and vessel wall thickness/lumina diameter was calculated. 
RESULTS AND CONCLUSION: The expression of p53 gene was found in donor hearts in Ad-p53 group at 5 days after operation. At 28 days after operation, the ratio of intima/media thickness and vessel wall thickness/lumina diameter were lower in Ad-p53 group than in Ad-LacZ and control group (P < 0.05). Ad-LacZ and control group show no significant difference (P > 0.05). The results confirmed that it is feasible to transfer target gene to the donor heart by intracoronarily injection. Wild-type P53 gene transfer to the donor heart can inhibit graft coronary artery intima hyperplasia and lumens narrow significantly.

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