Abstract:

BACKGROUND: Many studies focus on the change of conventional cell culture condition, a three-dimensional scaffold could be used to support high density culturing.
OBJECTIVE: To observe the proliferation of human bone marrow mesenchymal stem cells (hMSCs) in three-dimensional culture condition.
METHODS: The effect of basic fibroblast growth factor and lactic acid on the proliferation of hMSCs was measured. Based on the optimized condition, the growth and metabolism of hMSCs cultured in poly(ethylene terephthalate) were further investigated; Dyed by Cell tracker Green, the activity of hMSCs-poly(ethylene terephthalate) was imaged under a laser confocal microscope; the details of hMSCs-poly(ethylene terephthalate) were observed using scanning electron microscope.
RESULTS AND CONCLUSION: A low-does basic fibroblast growth factor (5 μg/L) and lactic acid (< 0.9 g/L) in culture medium displayed a significant promotion of the proliferation of hMSCs. Under the optimized condition, for the experimental group (with  5 μg/L basic fibroblast growth factor) and control (without 5 μg/L basic fibroblast growth factor), the expansion folds and specific growth rate were 15.29 and 0.136, 9.66 and 0.113, respectively. The amount of cells in the experimental group was increased 160% than that in control group. Observation of confocal laser microscopy and scanning electron microscope illustrated that the morphology and attachment of cells on poly(ethylene terephthalate) were stereoscopic, multi-level distributed, the unique structure of poly(ethylene terephthalate) three-dimensional scaffold provides cells with a larger growing space.