Abstract:

BACKGROUND: Previous studies have demonstrated that zhuanggu zhentong capsules (ZZC) can significantly inhibit the migrations and proliferations of breast cancer cell lines in vitro.
OBJECTIVE: Based on the previous experiment, to study the effects of ZZC on the transcriptions of rac-1 and PI3-K and pseudopodia of breast cancer cell lines MDA-MB-231 in vitro.
METHODS: SD rats were drenched 3-fold doses ZZC for 7 successively days, and then rat blood plasma were obtained to culture MDA-MB-231 cells for 24 hours, after that, the effects of drug blood plasma on pseudopodia of MDA-MB-231 cells and transcription of migrating relative genes rac-1 and PI3-K were observed under a scanning electronic microscope and Reverse transcriptase PCR (RT-PCR).
RESULTS AND CONCLUSION: The transcriptions of rac-1 and PI3-K genes and the development of pseudopodia of MDA-MB-231 cells were obviously inhibited when treated with the ZZC contained plasma at 5% and 1.25% concentration (P < 0.05). ZZC contained plasma at 0.63% concentration had no obvious inhibition on the transcriptions of rac-1 gene and the development of pseudopodia, but it obviously inhibited the transcriptions of PI3-K gene of MDA-MB-231 cells. It supposed that the inhibitive effects of ZZC contained plasma on MDA-MB-231 cells migrations in dose-dependent manner may be related to its inhibiting effects on the transcriptions of rac-1 and PI3-K genes and the development of pseudopodia.