Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (37): 6882-6886.doi: 10.3969/j.issn.1673-8225.2010.37.010

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Effect of epidermal growth factor combined with blood glucose control on wound healing in diabetes mellitus rats: Changes of fibroblast growth factor protein and its mRNA expressions

Zong Shou-kai, Liang Zi-qian, Ji Xue-liang   

  1. Department of Burns and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Online:2010-09-10 Published:2010-09-10
  • Contact: Liang Zi-qian, Professor, Master’s supervisor, Department of Burns and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China Liangzqian@yahoo.com
  • About author:Zong Shou-kai★, Studying for master’s degree, Department of Burns and Plastic Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • Supported by:

     the Science and Technology Foundation of Guangxi Zhuang Autonomous Region, No. 0542093*; Graduate Education Innovation Fund of Guangxi Zhuang Autonomous Region, No. 2009105981002 M198*

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Abstract:

BACKGROUND: Previous studies demonstrated that acute wound can result in down regulation of endogenic recombinant human epidermal growth factor (rhEGF) and lead to nonunion.
OBJECTIVE: To explore the effects of topical application of rhEGF combined with blood glucose control on the expression of fibroblast growth factor (FGF) on rat scald wound. 
METHODS: Wister diabetic rats were prepared for back deep Ⅱ degree scald models. The blood glucose levels of rats in the combination group were controlled to the levels of control group at 1 week before operation and were sprayed of rhEGF within 24 hours of scald. Rats in the rhEGF group only sprayed of rhEGF and the fasting blood glucose (FBG) were controlled in the FBG control group. The treatment in the control group was identical to the combination group. The healing rate of the wound was observed and expression of fibroblast growth factor (FGF) protein and mRNA were determined by immunohistochemistry and in situ hybridization at 1, 3, 5, 7, 11, 15 and 21 days after operation. 
RESULTS AND CONCLUSION: The expressions of FGF protein and mRNA in all rats were obviously increased in each group after scald, reached a peak at 5-7 and 7-11 days, respectively, in addition, the peak value of the combination and control groups were greater than that of the rhEGF and FBG control group (P < 0.05). The healing rates of the combination and control group were greater than that of the rhEGF and FBG control group at 7-21 days after scald (P < 0.05). The results demonstrated that external application of rhEGF combined with blood sugar control can accelerate obviously wound healing in diabetes mellitus rats, which may be related to the high expression of FGF.

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