Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (37): 6869-6872.doi: 10.3969/j.issn.1673-8225.2010.37.007

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Expression of interleukin 10 and tumor necrosis factor alpha in tissue engineered skins transplantation

Peng Yan1, Liu De-wu1, Yang Hui2, Lan Wei1, Zhong Qing-ling1   

  1. 1 Institute of Burn, First Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China; 2 Department of Immunology, Medical College of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Online:2010-09-10 Published:2010-09-10
  • Contact: Liu De-wu, Doctor, Professor, Chief physician, Doctoral supervisor, Institute of Burn, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China dewuliu@126.com
  • About author:Peng Yan★, Master, Technician-in-charge, Institute of Burn, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China
  • Supported by:

    the Science and Technology Planning Project of Health Bureau of Jiangxi Province, No. 20093037*; the Science and Technology Foundation of Nanchang University, No. 2009036*

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Abstract:

BACKGROUND: Interleukin 10 (IL-10) and tumor necrosis factor α (TNF-α) play an important role in allogeneic and xenogeneic skin graft rejection in local immune. The expression of TNF-α and IL-10 is unclear in the process of artificial skin graft in local skin tissue.

OBJECTIVE: To construct the artificial skin with epidermal stem cells and acellular dermal and to repair the rabbit full thickness dermal wounds, to observe the effect of wound repair and local skin tissue IL-10 and TNF-α expression.
METHODS: The in vitro cultured human epidermal stem cells or keratinocytes were incubated into acellular dermal matrix to construct tissue-engineered artificial skin. New Zealand White rabbits were prepared for back full thickness dermal wounds and randomly divided into 4 groups. Tissue-engineered skin grafts with epidermal stem cells, keratinocytes or acellular dermis were used to repair skin wounds. There was no treatment in the control group. The wound healing, local inflammatory response, and wound healing time were observed. The histomorphology of wound surface and the expressions of TNF-α and IL-10 at 7 days after operation were observed. 
RESULTS AND CONCLUSION: In the epidermal stem cell group, the local inflammatory response was slightly, without bleeding, empyema or necrosis. The wound healing time was shortened significantly compared with than of the keratinocyte group. There was IL-10 expression in all groups, especially strongest in the epidermal stem cell group, followed by keratinocyte group; the expression was weak in the acellular dermis and control groups. The TNF-α was expressed in all groups, which was strongest in the keratinocyte group, followed by epidermal stem cell group, but weak in the acellular dermis and control groups. The construction of the artificial skin with epidermal stem cells and acellular dermal can be used effectively to promote the repair of skin defect wound treatment. The expression changes of IL-10 and TNF-α in local skin tissue maybe one of its main mechanisms.

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