Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (37): 6857-6860.doi: 10.3969/j.issn.1673-8225.2010.37.004

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Dynamic mRNA expression of bone morphogenetic protein-2 and vascular endothelial growth factor in different regions of rat femoral nonunion

Xu Xiao-feng1, Li Yang1, Qian Dong1, Ma Peng2, Liu Xiao-ping1, Cui Xue-wen1   

  1. 1 Department of Orthopedics, 2 Department of Anesthesiology, Affiliated Hospital of Jiangsu University, Zhenjiang  212001, Jiangsu Province, China
  • Online:2010-09-10 Published:2010-09-10
  • About author:Xu Xiao-feng, Chief physician, Associate professor, Master’s supervisor, Department of Orthopedics, Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China andy.china@foxmail.com
  • Supported by:

    the Foundation for Society Development of Zhenjiang City, No. SH2002019*

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Abstract:

BACKGROUND: Bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) can promote new born formation, and elevate cure rate of bone nonunion.
OBJECTIVE: To observe the dynamic mRNA expression of BMP-2 and VEGF during restoration of rat femoral nonunion.
METHODS: SD rats were established femoral nonunion models and randomly divided into 7 groups, rats were sacrificed at 1, 3, 7, 14, 21, 28 and 35 days after model preparation. Wounds of rats in the control group were directly sutured without defects. The mRNA expression of BMP-2 and VEGF were detected using RT-PCT technique.
RESULTS AND CONCLUSION: The mRNA expression of BMP-2 and VEGF at the outer zone of injury were increased after injury, reached a peak at 7 days, and the decreased with time prolonged. However, the mRNA expression of BMP-2 and VEGF at the central zone of injury were delayed expressed and smaller than that of the outer zone. Low and delay expression of BMP-2 and VEGF in the central zone during the early stage may be an important reason for bone nonunion, thus, supplying extrinsic source of BMP-2 and VEGF in early stage may be contribute to the repairing of nonunion.

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