Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (21): 3985-3988.doi: 10.3969/j.issn.1673-8225.2010.21.045

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Degradable scaffolds combined with basic fibroblast growth factor for myocardial angiogenesis

Zhao Jian1, Cheng Zhao-yun1, Lü Feng2, Liu Tian-jun2, Liu Xiao-cheng3   

  1. 1 Department of Cardiovascular Surgery, Henan Provincial People’s Hospital, Zhengzhou  450003, Henan Province, China; 2 Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Tianjin  300193, China; 3 Department of Cardiovascular Surgery, TEDA International Cardiovascular Hospital, Tainjin  300457, China
  • Online:2010-05-21 Published:2010-05-21
  • About author:Zhao Jian☆, Doctor, Attending physician, Department of Cardiovascular Surgery, Henan Provincial People’s Hospital, Zhengzhou 450003, Henan Province, China doctorzhao66@126.com

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Abstract:

BACKGROUND: Studies have shown that basic fibroblast growth factor has effects on stimulating vessel regeneration and collateral reconstruction. However, administration was performed mostly by peripheral vein, left atrium or percutaneous coronary intervention, and it is difficult to achieve an effective therapeutic concentration in the local myocardium.
OBJECTIVE: Based on the property of poly(D, l-lactic-coglycolic acid) (PLGA), to investigate outcomes of inducing neovascularization in the myocardium in combination of basic fibroblast growth factor (bFGF) by ensuring target release of protein growth factor in local tissue.
METHODS: PLGA and bFGF were dissolved in dichloromethane. This liquid mixture was rolled into the form of a hollow tube (3.0 mm outer diameter, 2.8 mm inner diameter, 0.1 mm thick, 10 mm length) for further use. The middle third of the left anterior descending coronary artery of mini-swines was ligated, and the local myocardium became dark purple. After the successful establishment by abnormal regional wall motion in the cardiac apex at anterior wall using ultrasound, the mini-swines were assigned to channels and bare scaffolds (BS) group and channels and bFGF-incorporating scaffolds (FS) group. The scaffold was implanted in the myocardium using self-made hollow bit. At 6 weeks, the number of proliferative cells was quantified by immunohistochemical staining. New vessels were quantified utilizing Image-Pro Plus software package in both groups. Quantitative analysis of changes in mass defect percentage was performed by Emory Cardiac Toolbox software combined with single-photon-emission computed tomography.
RESULTS AND CONCLUSION: At 6 weeks, number of proliferative cells and the density of new vessels were significantly increased in the FS group compared with BS group (P < 0.001). Single-photon-emission computed tomography illustrates that MDP was significantly lower in the FS group compared with the BS group (P < 0.001). Results have suggested that PLGA scaffolds that incorporate bFGF were able to induce angiogenesis and enhance blood-flow perfusion.

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