Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (18): 3262-3266.doi: 10.3969/j.issn.1673-8225.2010.18.008

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Detction of urinary kidney injury molecule-1 for diagnosis of early graft function in kidney transplantation

Huo Wen-qian, Jin Feng-shuo, Nie Zhi-lin, Li Qian-sheng, Zhu Fang-qiang, Zhang Ke-qin   

  1. Department of Urology Surgery, Research Institute of Field Surgery, Daping Hospital, Third Military Medical University of Chinese PLA, Chongqing 400042, China
  • Online:2010-04-30 Published:2010-04-30
  • Contact: Zhang Ke-qin, Professor, Associate professor, Department of Urology Surgery, Research Institute of Field Surgery, Daping Hospital, Third Military Medical University of Chinese PLA, Chongqing 400042, China zhkq2000@sina.com
  • About author:Huo Wen-qian★, Master, Attending physician, Department of Urology Surgery, Research Institute of Field Surgery, Daping Hospital, Third Military Medical University of Chinese PLA, Chongqing 400042, China huowenqian@yahoo.com.cn
  • Supported by:

    Clinical Research Foundation of the Third Military Medical University of Chinese PLA, No. 2008XG182*

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Abstract:

BACKGROUND: Urinary kidney injury molecule-1 (KIM-1) has been proved to be a novel kidney-specific injury molecule as a marker for the diagnosis of acute renal ischemia injury, and KIM-1 participated in the progress of renal injury repair. However, no one reported the significance of its dynamic expression during the functional rehabilitation of renal graft.  
OBJECTIVE: To investigate the relations between urinary KIM-1 level and the early renal graft function in order to provide rational approaches for evaluating or predicting early renal graft function.
METHODS: The 46 patients were divided into 3 groups, including 22 cases of immediate graft function (IGF), 14 cases of slow graft function (SGF) and 10 cases of delayed graft function (DGF). The 24-hour urine specimen was collected every day for 2 weeks since the operation. The urinary KIM-1 content was detected by enzyme linked immunosorbent assay (ELISA), and at the same time the urinary and serum creatinine levels were detected. The diversity of urinary KIM-1 level was observed during the recovery of the graft function, and the clinical significance was evaluated by analyzing the correlation of urinary KIM-1 level and serum creatinine.
RESULTS AND CONCLUSION: At the first 2 days after kidney transplantation, the urinary KIM-1 levels were high and no significant difference was observed between the three groups (P < 0.05). Two days later, the urinary KIM-1 level descended quickly along with the descent of the serum creatinine in IGF and SGF groups; the urinary KIM-1 maintained high levels until the serum creatinine reached normally. In DGF group, the urinary KIM-1 decreased quickly to a low level after 2 days from operation, but it increased promptly 1 to 2 days before the recovery of graft function and kept a high level until the serum creatinine reached normally. This suggested that consecutive detection of urinary KIM-1 is useful for monitoring the early graft function after kidney transplantation, and high urinary KIM-1 may suggest the recovery of graft function.

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