Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (18): 3247-3251.doi: 10.3969/j.issn.1673-8225.2010.18.005

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Protective effect of hyperoxic fluid on rat liver transplantation

Liang Zhong-ping1, Lao Xue-jun2, He Wen-fang3, Ding Hong-wen2, Su Ze-xuan2   

  1. 1 Qingyuan People’s Hospital, Fifth Affiliated Hospital of Jinan University, Qingyuan  511500, Guangdong Province, China; 2 First Affiliated Hospital of Jinan University, Guangzhou   510630, Guangdong Province, China; 3 Medical College of Jinnan University, Guangzhou   510630, Guangdong Province, China
  • Online:2010-04-30 Published:2010-04-30
  • Contact: Su Ze-xuan, Professor, Doctoral supervisor, First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China suz2008@126.com
  • About author:Liang Zhong-ping☆, Doctor, Attending physician, Qingyuan People’s Hospital, Fifth Affiliated Hospital of Jinan University, Qingyuan 511500, Guangdong Province, China liangzp2006@126.com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. 04010486*; a Grant from Guangdong Provincial Health Department, No. A2004337*

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Abstract:

BACKGROUND: Organ for transplantation is insufficient, and primary transplant of nonfunction caused by perfusion cryopreservation occasionally occurs. It is clinically significant to reduce organ damage caused by perfusion preservation.
OBJECTIVE: To explore the protective effect of hyperoxic perfusion fluid on liver transplantation in rats.
METHODS: A total of 40 Wistar rats were randomly divided two groups (n = 20) and respectively poured with Ringer lactate solution or hyperoxic ringer lactate solution. Each group comprised equal number of donors and recipients to prepare liver, kidney, and pancreas transplantation models. Hyaluronic acid (HA), alanine aminotransferase (ALT) and CD8+CD28- T cells were compared between two groups at the end of perfusion, and 1st and 3rd days after liver transplantation. The acute rejection score of liver tissues were also compared after operation.
RESULTS AND CONCLUSION: The HA, ALT and CD8+CD28-T cells were no significantly different between two groups before operation (P > 0.05). The HA and ALT of hyperoxic ringer lactate solution group was significantly Ringer lactate solution group after liver transplant (P < 0.05), but the CD8+CD28- T cells were greater (P < 0.05). The acute rejection scores for liver in hyperoxia liquid group were significantly less than the common liquid group (P < 0.05). Results show that hyperoxic solution can attenuate ischemia/reperfusion injury and protect rats undergoing liver transplantation.

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