Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (7): 1257-1261.doi: 10.3969/j.issn.1673-8225.2010.07.027

Previous Articles     Next Articles

Astragalus root injection regulates type Ⅰ collagen expression of rat osteoblasts in vitro

Guo Hai-ling1, Wang Xiang2, Xu Yu2, Zhan Hong-sheng2, Zhao Yong-fang2   

  1. 1 Shanghai University of Traditional Chinese Medicine, Shanghai   201203, China; 2 Institute of Orthopedics and Traumatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai   201203, China
  • Online:2010-02-12 Published:2010-02-12
  • Contact: Zhao Yong-fang, Doctor, Associate professor, Institute of Orthopedics and Traumatology, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China zhao_dingding@126.com
  • About author:Guo Hai-ling, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China Ghl22007190@163.com
  • Supported by:

    the National and Shanghai Key Program of Traditional Chinese Medical Science of Orthopedics and Traumatology, No. 100508*, T0303*; the National Natural Science Foundation of China, No. 30371793*, 30500674*; the grant from Shanghai Science and Technology Commission, No. 08ZR1419400*, 08411950300*; Shanghai Rising-Star Program, No. 04qmx1434*; the Innovation Program of Shanghai Education Commission, No. 08YZ60*; a grant from Shanghai Shenkang Hospital Development Center, No. SHDC12007312*

PDF

807

Abstract:

BACKGROUND: Type Ⅰ collagen is a specific collagen secreted by in vitro cultured osteoblast, and the formed network is the basis of bone mineralization, which also reflects the ability of osteoblast bone formation. Studies have shown astragalus root increased osteoblast proliferation. However, the effect of astragalus root on improving type Ⅰ collagen expression of osteoblast remains poorly understood. 
OBJECTIVE: To evaluate the effect of astragalus root injection on the abilities of rat cranium-derived osteoblast proliferation and type Ⅰ collagen expression.
METHODS: Rat osteoblast was cultured in vitro and divided into control group (MEM culture solution containing calf serum) and astragalus root groups (different concentrations). The effect on osteoblast proliferation was evaluated on days 1, 3, 5, 7, and 9 by MTT method. Moreover, the expression of type Ⅰ collagen protein was observed after 6 hours of treatment with astragalus root injection using in cell western-blot method. In addition, the gene expression of COLLα1 was investigated by real-time PCR method.
RESULTS AND CONCLUSION: From days 3 to 9, the different concentrations of astragalus root injection improved osteoblast proliferation, respectively compared with control group (P < 0.05), and this ascending trend peaked on day 7. Different concentrations of astragalus root injection improved COLLα1mRNA expression, especially 15% astragalus root injection was the most effective. The type Ⅰ collagen protein expression of 15% and 10% astragalus root injection were significantly greater compared with the control group (P < 0.05). Astragalus root injection improved in vitro cultured osteoblast proliferation and type Ⅰ collagen secretion in a certain dose-effect manner.

CLC Number: