Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (7): 1248-1252.doi: 10.3969/j.issn.1673-8225.2010.07.025

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Differential expression of OX40L in C57BL/6 and BALB/c mouse tissues

Han Xue-fu, Wang Fei, Zhu Shu-shu, Xu Jin-dan, Zhang Ji-nan, Yang Di   

  1. Institute of Cardiovascular Disease, First Affiliated Hospital of Nanjing Medical University, Nanjing  210029, Jiangsu Province, China
  • Online:2010-02-12 Published:2010-02-12
  • Contact: Yang Di, Researcher, Doctoral supervisor, Institute of Cardiovascular Disease, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China dyang@njmu.edu.cn
  • About author:Han Xue-fu★, Studying for master’s degree, Institute of Cardiovascular Disease, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China xf-han@hotmail.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30770894*; Advanced Training Fund for Medicine of Jiangsu Province, No. RC2007037*

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Abstract:

BACKGROUND: Previous studies found that C57BL/6 mouse was susceptible to atherosclerosis, while BALB/c mouse was resistant to atherosclerosis. The stenosis of the culprit vessel and the severity of myocardial infarction were correlated to the levels of OX40L expression. Whether OX40L has differential expression between C57BL/6 and BALB/c mouse was not identified.
OBJECTIVE: To observe the differential expression of OX40L mRNA and protein in C57BL/6 and BALB/c mouse.
METHODS: Total RNA and protein were extracted by Trizol and RIPA Buffer from heart, brain, kidney, skeletal muscle and spleen tissues of C57BL/6 and BALB/c mice. RT-PCR and Western Blot were used to detect OX40L mRNA and protein expression in heart, brain, kidney, spleen and skeletal muscle of two kinds of mice. The differential expression of OX40L mRNA and protein between C57BL/6 and BALB/c mice was observed.
RESULTS AND CONCLUSION: RT-PCR results showed that the mRNA expression level of OX40L in heart of C57BL/6 mice mouse was significantly higher than BALB/c mice (P < 0.05); the mRNA expression level of OX40L in spleen of BALB/c mice was significantly higher than C57BL/6 mice (P < 0.05). There were no significant differences in the brain, kidney and skeletal muscle between these two strains. The results of Western Blot showed that the protein expression level of OX40L in heart, brain and kidney of C57BL/6 mice were significantly higher than BALB/c mice (P < 0.05). There were no significant differences in skeletal muscle and spleen between these two strains. The OX40L mRNA transcription level in heart was higher in C57BL/6 mouse than BALB/c mouse, while the expression in spleen was lower than the latter. The OX40L protein levels in C57BL/6 mouse heart, brain and kidney were higher than BALB/c mouse. The differences of OX40L expression between the two strains of mice indicated that OX40L may promote to C57BL/6 mouse susceptible to atherosclerosis.　

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