Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (7): 1231-1234.doi: 10.3969/j.issn.1673-8225.2010.07.021

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Bioinformatic analysis of streptococcus mutans thrS gene and construction of homologous recombinant plasmids

Liu Xiao-di1, Duan Jin2, Guo Li-hong1   

  1. 1 Peking University School & Hospital of Stomatology, Beijing  100081, China; 2 Huzhou Teachers College, Huzhou  313000,  Zhejiang Province, China
  • Online:2010-02-12 Published:2010-02-12
  • Contact: Guo Li-hong, Doctor, Associate professor, Peking University School & Hospital of Stomatology, Beijing 100081, China guo_lihong2008@yahoo.com.cn
  • About author:Liu Xiao-di★, Master, Research assistant, Peking University School & Hospital of Stomatology, Beijing 100081, China kqlxd@163.com
  • Supported by:

    the Natural Science Foundation of Beijing, No. 7042035*

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Abstract:

BACKGROUND: Streptococcus mutans (S. mutans) is generally considered to be the principal aetiological agent for dental caries. thrS gene may relate to the virulence of S. mutans involved in the adherence, acidogenicity and acidodurance.
OBJECTIVE: To investigate the conservation status of the thrS gene of S. mutans and to construct the homologous recombinant plasmid.
METHODS: Southern Blot was used to analyze the distribution of thrS gene in S. mutans. The upstream and downstream sequences of thrS gene were cloned respectively into multiple cloning sites of suicide plasmid pFW5 to construct the recombinant plasmid.
RESULTS AND CONCLUSION: The thrS gene was conserved in 6 strains of S. mutans in this test. By PCR analysis and enzyme digesting, it was confirmed that S. mutans thrS gene homologous recombinant plasmid was successfully constructed, which can be used in future research of construction of thrS -negative mutans of S. mutans strain UA159

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