Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (7): 1174-1178.doi: 10.3969/j.issn.1673-8225.2010.07.008

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Construction of tissue-engineered skin using basic fibroblast growth factor gene transfected bone marrow mesenchymal stem cells combined with small intestinal submucosa

Wang Shao-yun1, Yang Hao1, Zhu Xiao-song1, Li Qing1, Li Shi-he1, Dong Jian2, Wu Di1   

  1. 1 Department of Orthopedics, 2 Centre of Biotherapy, First Affiliated Hospital, Kunming Medical College, Kunming  650032, Yunnan Province, China
  • Online:2010-02-12 Published:2010-02-12
  • Contact: Wu Di, Doctor, Attending physician, Department of Orthopedics, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China
  • About author:Wang Shao-yun☆, Studying for doctorate, Physician, Department of Orthopedics, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China reinhard007550@163.com
  • Supported by:

    the Special Foundation of Yunnan Provincial Science and Technology Bureau & Kunming Medical College, No. 2007C0002R*

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Abstract:

BACKGROUND: Studies demonstrated that small intestinal submucosa (SIS) had no immunogenicity, which can not lead to rejection following transplantation, thus, this is an ideal skin substitutes for natural skin.  
OBJECTIVE: Basic fibroblast growth factor (bFGF) gene was transfected into bone marrow mesenchymal stem cells (BMSCs), and combined the cells with SIS to construct tissue-engineered skin. 
METHODS: BMSCs were obtained from Japanese big-eared rabbits, and in vitro cultured. Then the subcultured BMSCs were transfected by pCDNA3.1 plasmid, followed by incubation on swine SIS to construct the tissue-engineered skin. The growth of cells and phenotype of BMSCs were detected by flow cytometry. In addition, the result of transfecting BMSCs with pCDNA-bFGF vector was measured by Western blot, and the structure of tissue-engineered skin was observed.
RESULTS AND CONCLUSION: After passaged, BMSCs were grown quickly with long-fusiform shape. The cells were positive expressed CD90 and CD44, but negative expressed CD45. bFGF had been transfected into BMSCs, and stable expressed. The transfected BMSCs grew well in SIS. By this method, tissue-engineered skin can be constructed in vitro.

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