Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (5): 824-827.doi: 10.3969/j.issn.1673-8225.2010.05.016

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Effects of cyclosporine combined with transforming growth factor beta 1 plasmid on rat immunological reaction following liver transplantation

Zhang Yan, Chen Xi-hai, Ji Yan-chao, Zhai Zhe, Wu Bo   

  1. Department of General Surgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin  150001, Heilongjiang Province, China
  • Online:2010-01-29 Published:2010-01-29
  • Contact: Wu Bo, Professor, Master’s supervisor, Department of General Surgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China xinxin9129@126.com
  • About author:Zhan Yan★, Master, Department of General Surgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China jbjsbr@126.com
  • Supported by:

     the Tackle Key Program of Heilongjiang Province, No. 2010G0252-00*; Program of Heilongjiang Educational Committee, No. 11541147*

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Abstract:

BACKGROUND: Most patients who underwent liver transplantation would suffer acute rejection or transplanted liver failure resulted by chronic rejection, therefore, inducing specific immune tolerance via varied pathways is the ideal method to solve this problem.

OBJECTIVE: To treat rat transplanted liver by injecting transforming growth factor β1 (TGF-β1) plasmid, and to analyze the relationship between TGF-β1 and allograft rejection from gene level.
METHODS: A total of 30 male, Wistar rats were served as allogenic liver donors, and 10 male, SD rats served as syngeneic donors Totally 40 male SD rats were served as liver recipients, and divided into 4 groups by order number table: allogenic transplantation, syngeneic transplantation, ciclosporin, and ciclosporin plus TGF-β1 groups. In each group, rat orthotopic liver transplantation model was established by modified Kamada and improved two-cuff technique. After modeling, rats were received cyclosporine 1-5 days in the cyclosporine group, or intraperitoneal injected ciclosporin for 1-5 days, combined with TGF-β1 plasmid 0-2 days in the cyclosporine plus TGF-β1 group. No intervention was performed in the other groups. The survival time of rats were recorded, and the pathological changes was detected at days 3, 7, 14, 21, and 28 after transplantation, then the mixed lymphocyte culture was performed..

RESULTS AND CONCLUSION: The survival time of rats in syngeneic transplantation group and cyclosporine plus TGF-β1 group was more than 60 days, which was obviously greater than that of allogenic transplantation and cyclosporine groups (P < 0.05). The histopathologic slide showed that there was moderate and severe acute rejection, with evident intrahepatic inflammatory cell infiltration in the allogenic transplantation and cyclosporine groups. Few rejections were observed in the syngeneic transplantation group, which was close to the normal lever tissues. Mixed lymphocyte culture of the cyclosporine plus TGF-β1 group was superior to the syngeneic transplantation group or cyclosporine group (P < 0.05). The results demonstrated that cyclosporine combined with local injection of TGF-β1 plasmid can relieve post-transplant immune rejection.

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