Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (5): 818-823.doi: 10.3969/j.issn.1673-8225.2010.05.015

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Correlation between total bile acid/phospholipid ratio and relative warm ischemia injury to the biliary tract in rat liver transplantation

Ren Xuan-lei, Zhao Hong-feng, Zhang Guo-wei, Wang Yu, Zhou Jie　   

  1. Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou  510515, Guangdong Province, China
  • Online:2010-01-29 Published:2010-01-29
  • Contact: Zhou Jie, Master, Professor, Chief physician, Doctoral supervisor, Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China jacky@fimmu.com
  • About author:Ren Xuan-lei☆, Doctor, Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China renxuanlei1202@126.com
  • Supported by:

    Science and Technology Planning Project of Guangdong Province, No. 2007B031514004*

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Abstract:

BACKGROUND: Studies have shown that long time of warm ischemia or cold preservation would injury the biliary tract in liver transplantation. However, whether relative warm ischemia (RWI) of biliary tract would result in bile component changes is unclearly.
OBJECTIVE: To establish auto-liver transplantation bile ducts RWI models, observe the effects of RWI on the bile salts and phospholipid concentration secreted by the donor liver, and to study the correlation between the total bile salt∕phospholipid ratio (TBA∕PL ratio) and biliary tract injury.
METHODS: A total of 32 SD rats were selected for auto-liver transplantation models with bile ducts RWI, and the rats were randomly divided into 4 groups (n=8). In Group Ⅰ (sham operation group), rats only received liver dissociation without any cold reperfusion. The RWI time of GroupⅡ, Ⅲ, and Ⅳ were 0 minutes, 30 minutes and 60 minutes, respectively. The concentration of TBA in bile was measured with enzymatic cycling assay, and PL with enzymic colorimetric. Pathological observation with light microscope and ultrastructural observation with transmission electron microscope were performed on the hilar bile duct. The endothelial cell apoptosis was detected with TUNEL assay. The correlation between TBA∕PL ratio and biliary injury was analyzed.
RESULTS AND CONCLUSION: One rat died, the other 31 rats were included in the final analysis. RWI could change the composition of bile secreted by donor liver, raise the TBA∕PL ratio, and increase the bile toxicity. These changes had a positive correlation to RWI time, and the changes were obviously with time prolonged. In addition, the changes are closely related to the biliary tract injury. This study shows an important mechanism of the biliary tract injury caused by RWI-injury.

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