Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (28): 5157-5160.doi: 10.3969/j.issn.1673-8225.2011.28.007

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Estrogen receptor beta inhibits the proliferation and differentiation of osteoblasts in mice

Lu Shi-jin, Zhang Hong-qi, Gao Qi-le, Guo Chao-feng, Tang Ming-xing   

  1. Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China
  • Received:2011-04-09 Revised:2011-05-18 Online:2011-07-09 Published:2011-07-09
  • Contact: Zhang Hong-qi, Professor, Lotus scholar, Doctoral supervisor, Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China zhq9996@163.com
  • About author:Lu Shi-jin☆, Studying for doctorate, Attending physician, Department of Spine Surgery, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China jzwklu@yahoo.com.cn
  • Supported by:

    Scientific Research Innovation Program  of Human Postgraduates, No.CX2010B101

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Abstract:

BACKGROUND: Estrogen receptors (ERs) are expressed in cell components related to bone formation and resorption.
OBJECTIVE: To investigate the regulatory effects of Erβ on osteoblast proliferation and differentiation.
METHODS: The mouse osteoblastic cell lines MC3T3-E1 were divided into 3 groups: experimental group (transfected ERβRNAi vector), negative control (transfected ERL RNAi vector), and blank control (no transfection). Three groups of cells were cultured under the same conditions. In each group, cell growth curve was drawn using MTT; and the cell cycle was analyzed by flow cytometry.
RESULTS AND CONCLUSION: The cell proliferation in the experimental group was more significantly enhanced than in the negative control group or blank control group. The percentage of cells at G1-phase in the experimental group was significantly less than the other groups, while the percentage of cells at S-phase and G2-phase was significantly higher than the other groups (P < 0.05). According to test results of the ERβ silence, we can infer that the ERβ may inhibit the proliferation and differentiation of osteoblasts.

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